2wwa: Difference between revisions

Latest revision as of 10:18, 9 May 2024

Cryo-EM structure of idle yeast Ssh1 complex bound to the yeast 80S ribosome

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Structural highlights

2wwa is a 10 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSH1_YEAST Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.

Structure of monomeric yeast and Mammalian sec61 complexes interacting with the translating ribosome.,Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R Science. 2009 Dec 4;326(5958):1369-73. Epub 2009 Oct 29. PMID:19933108^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R. Structure of monomeric yeast and Mammalian sec61 complexes interacting with the translating ribosome. Science. 2009 Dec 4;326(5958):1369-73. Epub 2009 Oct 29. PMID:19933108

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R. Structure of monomeric yeast and Mammalian sec61 complexes interacting with the translating ribosome. Science. 2009 Dec 4;326(5958):1369-73. Epub 2009 Oct 29. PMID:19933108

[1]

@@ Line 3: / Line 3: @@
 <SX load='2wwa' size='340' side='right' viewer='molstar' caption='[[2wwa]], [[Resolution|resolution]] 8.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wwa]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WWA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wwa]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WWA FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1k5y|1k5y]], [[1s1i|1s1i]], [[2wwb|2wwb]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.9&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wwa OCA], [https://pdbe.org/2wwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wwa RCSB], [https://www.ebi.ac.uk/pdbsum/2wwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wwa ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RL25_YEAST RL25_YEAST]] This protein binds to a specific region on the 26S rRNA. [[https://www.uniprot.org/uniprot/SC6B2_YEAST SC6B2_YEAST]] Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import. [[https://www.uniprot.org/uniprot/SC61G_YEAST SC61G_YEAST]] Part of the Sec61 complex, which is the major component of channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2. Also part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein ER import.[HAMAP-Rule:MF_00422] [[https://www.uniprot.org/uniprot/RL4B_YEAST RL4B_YEAST]] Participates in the regulation of the accumulation of its own mRNA. [[https://www.uniprot.org/uniprot/SSH1_YEAST SSH1_YEAST]] Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import.
+[https://www.uniprot.org/uniprot/SSH1_YEAST SSH1_YEAST] Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 44: / Line 44: @@
 [[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Armache, J P]]
+[[Category: Armache JP]]
-[[Category: Becker, T]]
+[[Category: Becker T]]
-[[Category: Beckmann, R]]
+[[Category: Beckmann R]]
-[[Category: Berninghausen, O]]
+[[Category: Berninghausen O]]
-[[Category: Bhushan, S]]
+[[Category: Bhushan S]]
-[[Category: Funes, S]]
+[[Category: Funes S]]
-[[Category: Gilmore, R]]
+[[Category: Gilmore R]]
-[[Category: Gumbart, J]]
+[[Category: Gumbart J]]
-[[Category: Jarasch, A]]
+[[Category: Jarasch A]]
-[[Category: Jossinet, F]]
+[[Category: Jossinet F]]
-[[Category: Mandon, E]]
+[[Category: Mandon E]]
-[[Category: Mielke, T]]
+[[Category: Mielke T]]
-[[Category: Schulten, K]]
+[[Category: Schulten K]]
-[[Category: Westhof, E]]
+[[Category: Westhof E]]
-[[Category: Cotranslational protein translocation]]
-[[Category: Endoplasmic reticulum]]
-[[Category: Isopeptide bond]]
-[[Category: Membrane]]
-[[Category: Phosphoprotein]]
-[[Category: Protein conducting channel]]
-[[Category: Protein exit tunnel]]
-[[Category: Protein transport]]
-[[Category: Ribonucleoprotein]]
-[[Category: Ribosomal protein]]
-[[Category: Ribosome]]
-[[Category: Rna-binding]]
-[[Category: Rrna-binding]]
-[[Category: Signal sequence]]
-[[Category: Translocation]]
-[[Category: Transmembrane]]
-[[Category: Transport]]

2wwa: Difference between revisions

Latest revision as of 10:18, 9 May 2024

Cryo-EM structure of idle yeast Ssh1 complex bound to the yeast 80S ribosome

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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