6xi3

Crystal structure of tetra-tandem repeat in extending region of large adhesion protein

spinqualitylabelsall models 6xi3, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xi3 is a 1 chain structure with sequence from Marinobacter nauticus ATCC 49840. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: CA, CL, EDO, EDT, NA, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function H8W6K8_MARN1 Publication Abstract from PubMed RTX adhesins are long, multi-domain proteins present on the outer membrane of many Gram-negative bacteria. From this vantage point, adhesins use their distal ligand-binding domains for surface attachment leading to biofilm formation. To expand the reach of the ligand-binding domains, RTX adhesins maintain a central extender region of multiple tandem repeats, which makes up most of the proteins' large molecular weight. Alignments of the 10-15-kDa extender domains show low sequence identity between adhesins. Here we have produced and structurally characterized protein constructs of four tandem repeats (tetra-tandemers) from two different RTX adhesins. In comparing the tetra-tandemers to each other and already solved structures from Marinomonas primoryensis and Salmonella enterica, the extender domains fold as diverse beta-sandwich structures with widely differing calcium contents. However, all the tetra-tandemers have at least one calcium ion coordinated in the linker region between beta-sandwich domains whose role appears to be the rigidification of the extender region to help the adhesin extend its reach. Essential role of calcium in extending RTX adhesins to their target.,Vance TDR, Ye Q, Conroy B, Davies PL J Struct Biol X. 2020 Sep 8;4:100036. doi: 10.1016/j.yjsbx.2020.100036., eCollection 2020. PMID:32984811 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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