6yrf: Difference between revisions

Latest revision as of 10:21, 22 May 2024

Vip3Bc1 tetramer

Structural highlights

6yrf is a 4 chain structure with sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A290WPI2_BACTU

Publication Abstract from PubMed

Insect pests are a major cause of crop losses worldwide, with an estimated economic cost of $470 billion annually. Biotechnological tools have been introduced to control such insects without the need for chemical pesticides; for instance, the development of transgenic plants harbouring genes encoding insecticidal proteins. The Vip3 (vegetative insecticidal protein 3) family proteins from Bacillus thuringiensis convey toxicity to species within the Lepidoptera, and have wide potential applications in commercial agriculture. Vip3 proteins are proposed to exert their insecticidal activity through pore formation, though to date there is no mechanistic description of how this occurs on the membrane. Here we present cryo-EM structures of a Vip3 family toxin in both inactive and activated forms in conjunction with structural and functional data on toxin-membrane interactions. Together these data demonstrate that activated Vip3Bc1 complex is able to insert into membranes in a highly efficient manner, indicating that receptor binding is the likely driver of Vip3 specificity.

Cryo-EM structures of an insecticidal Bt toxin reveal its mechanism of action on the membrane.,Byrne MJ, Iadanza MG, Perez MA, Maskell DP, George RM, Hesketh EL, Beales PA, Zack MD, Berry C, Thompson RF Nat Commun. 2021 May 14;12(1):2791. doi: 10.1038/s41467-021-23146-4. PMID:33990582^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Byrne MJ, Iadanza MG, Perez MA, Maskell DP, George RM, Hesketh EL, Beales PA, Zack MD, Berry C, Thompson RF. Cryo-EM structures of an insecticidal Bt toxin reveal its mechanism of action on the membrane. Nat Commun. 2021 May 14;12(1):2791. doi: 10.1038/s41467-021-23146-4. PMID:33990582 doi:https://dx.doi.org/10.1038/s41467-021-23146-4

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OCA

[1] Byrne MJ, Iadanza MG, Perez MA, Maskell DP, George RM, Hesketh EL, Beales PA, Zack MD, Berry C, Thompson RF. Cryo-EM structures of an insecticidal Bt toxin reveal its mechanism of action on the membrane. Nat Commun. 2021 May 14;12(1):2791. doi: 10.1038/s41467-021-23146-4. PMID:33990582 doi:https://dx.doi.org/10.1038/s41467-021-23146-4

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6yrf' size='340' side='right'caption='[[6yrf]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6yrf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YRF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6yrf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YRF FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vip3Bc1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428 "Bacillus cereus var. thuringiensis" Smith et al. 1952])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yrf OCA], [https://pdbe.org/6yrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yrf RCSB], [https://www.ebi.ac.uk/pdbsum/6yrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yrf ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A290WPI2_BACTU A0A290WPI2_BACTU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
+[[Category: Bacillus thuringiensis]]
 [[Category: Large Structures]]
-[[Category: Beales, P A]]
+[[Category: Arribas Perez M]]
-[[Category: Berry, C]]
+[[Category: Beales PA]]
-[[Category: Byrne, M J]]
+[[Category: Berry C]]
-[[Category: George, R M]]
+[[Category: Byrne MJ]]
-[[Category: Hesketh, E L]]
+[[Category: George RM]]
-[[Category: Iadanza, M I]]
+[[Category: Hesketh EL]]
-[[Category: Maskell, D P]]
+[[Category: Iadanza MI]]
-[[Category: Perez, M Arribas]]
+[[Category: Maskell DP]]
-[[Category: Thompson, R F]]
+[[Category: Thompson RF]]
-[[Category: Zack, M D]]
+[[Category: Zack MD]]
-[[Category: Bt toxin]]
-[[Category: Toxin]]
-[[Category: Vip3]]

6yrf: Difference between revisions

Latest revision as of 10:21, 22 May 2024

Vip3Bc1 tetramer

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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