8wmc: Difference between revisions
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==Cryo-EM structure of DiCas7-11-crRNA in complex with regulator== | |||
<StructureSection load='8wmc' size='340' side='right'caption='[[8wmc]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8wmc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WMC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wmc OCA], [https://pdbe.org/8wmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wmc RCSB], [https://www.ebi.ac.uk/pdbsum/8wmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wmc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A401FT52_9BACT A0A401FT52_9BACT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CRISPRâCas7-11 is a Type III-E CRISPR-associated nuclease that functions as a potent RNA editing tool. Tetratrico-peptide repeat fused with Cas/HEF1-associated signal transducer (TPR-CHAT) acts as a regulatory protein that interacts with CRISPR RNA (crRNA)-bound Cas7-11 to form a CRISPR-guided caspase complex (Craspase). However, the precise modulation of Cas7-11's nuclease activity by TPR-CHAT to enhance its utility requires further study. Here, we report cryo-electron microscopy (cryo-EM) structures of Desulfonema ishimotonii (Di) Cas7-11-crRNA, complexed with or without the full length or the N-terminus of TPR-CHAT. These structures unveil the molecular features of the Craspase complex. Structural analysis, combined with in vitro nuclease assay and electrophoretic mobility shift assay, reveals that DiTPR-CHAT negatively regulates the activity of DiCas7-11 by preventing target RNA from binding through the N-terminal 65 amino acids of DiTPR-CHAT (DiTPR-CHAT(NTD)). Our work demonstrates that DiTPR-CHAT(NTD) can function as a small unit of DiCas7-11 regulator, potentially enabling safe applications to prevent overcutting and off-target effects of the CRISPRâCas7-11 system. | |||
Structural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.,Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X Signal Transduct Target Ther. 2024 May 13;9(1):111. doi: , 10.1038/s41392-024-01821-4. PMID:38735995<ref>PMID:38735995</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Ma | <div class="pdbe-citations 8wmc" style="background-color:#fffaf0;"></div> | ||
[[Category: Tang | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Desulfonema ishimotonii]] | |||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Ma HY]] | |||
[[Category: Tang XD]] | |||
Latest revision as of 18:08, 29 May 2024
Cryo-EM structure of DiCas7-11-crRNA in complex with regulator
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