2f33: Difference between revisions

Latest revision as of 18:55, 29 May 2024

NMR solution structure of Ca2+-loaded calbindin D28K

Structural highlights

2f33 is a 1 chain structure with sequence from Rattus norvegicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CALB1_RAT Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Calbindin-D(28K) is a Ca2+-binding protein, performing roles as both a calcium buffer and calcium sensor. The NMR solution structure of Ca2+-loaded calbindin-D(28K) reveals a single, globular fold consisting of six distinct EF-hand subdomains, which coordinate Ca2+ in loops on EF1, EF3, EF4 and EF5. Target peptides from Ran-binding protein M and myo-inositol monophosphatase, along with a new target from procaspase-3, are shown to interact with the protein on a surface comprised of alpha5 (EF3), alpha8 (EF4) and the EF2-EF3 and EF4-EF5 loops. Fluorescence experiments reveal that calbindin-D(28K) adopts discrete hydrophobic states as it binds Ca2+. The structure, binding interface and hydrophobic characteristics of Ca2+-loaded calbindin-D(28K) provide the first detailed insights into how this essential protein may function. This structure is one of the largest high-resolution NMR structures and the largest monomeric EF-hand protein to be solved to date.

Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D(28K).,Kojetin DJ, Venters RA, Kordys DR, Thompson RJ, Kumar R, Cavanagh J Nat Struct Mol Biol. 2006 Jul;13(7):641-7. Epub 2006 Jun 25. PMID:16799559^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kojetin DJ, Venters RA, Kordys DR, Thompson RJ, Kumar R, Cavanagh J. Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D(28K). Nat Struct Mol Biol. 2006 Jul;13(7):641-7. Epub 2006 Jun 25. PMID:16799559 doi:https://dx.doi.org/10.1038/nsmb1112

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Kojetin DJ, Venters RA, Kordys DR, Thompson RJ, Kumar R, Cavanagh J. Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D(28K). Nat Struct Mol Biol. 2006 Jul;13(7):641-7. Epub 2006 Jun 25. PMID:16799559 doi:https://dx.doi.org/10.1038/nsmb1112

[1]

@@ Line 1: / Line 1: @@
 ==NMR solution structure of Ca2+-loaded calbindin D28K==
-<StructureSection load='2f33' size='340' side='right'caption='[[2f33]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2f33' size='340' side='right'caption='[[2f33]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2f33]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F33 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2f33]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F33 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Calb1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f33 OCA], [https://pdbe.org/2f33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f33 RCSB], [https://www.ebi.ac.uk/pdbsum/2f33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f33 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CALB1_RAT CALB1_RAT]] Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
+[https://www.uniprot.org/uniprot/CALB1_RAT CALB1_RAT] Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Buffalo rat]]
 [[Category: Large Structures]]
-[[Category: Cavanagh, J]]
+[[Category: Rattus norvegicus]]
-[[Category: Kojetin, D J]]
+[[Category: Cavanagh J]]
-[[Category: Kordys, D R]]
+[[Category: Kojetin DJ]]
-[[Category: Kumar, R]]
+[[Category: Kordys DR]]
-[[Category: Thompson, R J]]
+[[Category: Kumar R]]
-[[Category: Venters, R A]]
+[[Category: Thompson RJ]]
-[[Category: Ca2+-binding]]
+[[Category: Venters RA]]
-[[Category: Ef-hand]]
-[[Category: Metal binding protein]]

2f33: Difference between revisions

Latest revision as of 18:55, 29 May 2024

NMR solution structure of Ca2+-loaded calbindin D28K

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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