7kwg: Difference between revisions
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==== | ==Staphylococcus aureus 30S ribosomal subunit in presence of spermidine== | ||
<StructureSection load='7kwg' size='340' side='right'caption='[[7kwg]]' scene=''> | <StructureSection load='7kwg' size='340' side='right'caption='[[7kwg]], [[Resolution|resolution]] 3.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7kwg]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWG FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwg OCA], [https://pdbe.org/7kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwg RCSB], [https://www.ebi.ac.uk/pdbsum/7kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwg ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.75Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwg OCA], [https://pdbe.org/7kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwg RCSB], [https://www.ebi.ac.uk/pdbsum/7kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwg ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/RS20_STAA8 RS20_STAA8] Binds directly to 16S ribosomal RNA. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cryo-electron microscopy is now used as a method of choice in structural biology for studying protein synthesis, a process mediated by the ribosome machinery. In order to achieve high-resolution structures using this approach, one needs to obtain homogeneous and stable samples, which requires optimization of ribosome purification in a species-dependent manner. This is especially critical for the bacterial small ribosomal subunit that tends to be unstable in the absence of ligands. Here, we report a protocol for purification of stable 30 S from the Gram-positive bacterium Staphylococcus aureus and its cryo-EM structures: in presence of spermidine at a resolution ranging between 3.4 and 3.6 A and in its absence at 5.3 A. Using biochemical characterization and cryo-EM, we demonstrate the importance of spermidine for stabilization of the 30 S via preserving favorable conformation of the helix 44. | |||
Stabilization of Ribosomal RNA of the Small Subunit by Spermidine in Staphylococcus aureus.,Belinite M, Khusainov I, Soufari H, Marzi S, Romby P, Yusupov M, Hashem Y Front Mol Biosci. 2021 Nov 18;8:738752. doi: 10.3389/fmolb.2021.738752. , eCollection 2021. PMID:34869582<ref>PMID:34869582</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7kwg" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] | ||
[[Category: Belinite M]] | |||
[[Category: Hashem Y]] | |||
[[Category: Khusainov I]] | |||
[[Category: Marzi S]] | |||
[[Category: Romby P]] | |||
[[Category: Yusupov M]] | |||
Latest revision as of 19:36, 29 May 2024
Staphylococcus aureus 30S ribosomal subunit in presence of spermidine
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