7lis: Difference between revisions

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<StructureSection load='7lis' size='340' side='right'caption='[[7lis]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
<StructureSection load='7lis' size='340' side='right'caption='[[7lis]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7lis]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIS FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.96&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lis OCA], [https://pdbe.org/7lis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lis RCSB], [https://www.ebi.ac.uk/pdbsum/7lis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lis ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lis OCA], [https://pdbe.org/7lis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lis RCSB], [https://www.ebi.ac.uk/pdbsum/7lis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lis ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/MARIT_THEMA MARIT_THEMA]] Protease that exhibits activity toward chymotrypsin and trypsin substrates. May have antibacterial activity.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein cages are a common architectural motif used by living organisms to compartmentalize and control biochemical reactions. While engineered protein cages have featured in the construction of nanoreactors and synthetic organelles, relatively little is known about the underlying molecular parameters that govern stability and flux through their pores. In this work, we systematically designed 24 variants of the Thermotoga maritima encapsulin cage, featuring pores of different sizes and charges. Twelve pore variants were successfully assembled and purified, including eight designs with exceptional thermal stability. While negatively charged mutations were better tolerated, we were able to form stable assemblies covering a full range of pore sizes and charges, as observed in seven new cryo-EM structures at 2.5- to 3.6-A resolution. Molecular dynamics simulations and stopped-flow experiments revealed the importance of considering both pore size and charge, together with flexibility and rate-determining steps, when designing protein cages for controlling molecular flux.
Pore structure controls stability and molecular flux in engineered protein cages.,Adamson LSR, Tasneem N, Andreas MP, Close W, Jenner EN, Szyszka TN, Young R, Cheah LC, Norman A, MacDermott-Opeskin HI, O'Mara ML, Sainsbury F, Giessen TW, Lau YH Sci Adv. 2022 Feb 4;8(5):eabl7346. doi: 10.1126/sciadv.abl7346. Epub 2022 Feb 4. PMID:35119930<ref>PMID:35119930</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7lis" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Adamson, L]]
[[Category: Adamson L]]
[[Category: Andreas, M P]]
[[Category: Andreas MP]]
[[Category: Close, W]]
[[Category: Close W]]
[[Category: Giessen, T]]
[[Category: Giessen T]]
[[Category: Lau, Y H]]
[[Category: Lau YH]]
[[Category: Tasneem, N]]
[[Category: Tasneem N]]
[[Category: Encapsulin]]
[[Category: Nanocompartment]]
[[Category: Virus like particle]]
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