8vci: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vci OCA], [https://pdbe.org/8vci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vci RCSB], [https://www.ebi.ac.uk/pdbsum/8vci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vci ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vci OCA], [https://pdbe.org/8vci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vci RCSB], [https://www.ebi.ac.uk/pdbsum/8vci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vci ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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== Publication Abstract from PubMed == | |||
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshift stimulatory element (FSE) is necessary for programmed -1 ribosomal frameshifting (-1 PRF) and optimized viral efficacy. The FSE has an abundance of context-dependent alternate conformations, but two of the structures most crucial to -1 PRF are an attenuator hairpin and a three-stem H-type pseudoknot structure. A crystal structure of the pseudoknot alone features three RNA stems in a helically stacked linear structure, whereas a 6.9 A cryo-EM structure including the upstream heptameric slippery site resulted in a bend between two stems. Our previous research alluded to an extended upstream multibranch loop that includes both the attenuator hairpin and the slippery site-a conformation not previously modeled. We aim to provide further context to the SARS-CoV-2 FSE via computational and medium resolution cryo-EM approaches, by presenting a 6.1 A cryo-EM structure featuring a linear pseudoknot structure and a dynamic upstream multibranch loop. | |||
Structure of the SARS-CoV-2 Frameshift Stimulatory Element with an Upstream Multibranch Loop.,Peterson JM, Becker ST, O'Leary CA, Juneja P, Yang Y, Moss WN Biochemistry. 2024 May 21;63(10):1287-1296. doi: 10.1021/acs.biochem.3c00716. , Epub 2024 May 10. PMID:38727003<ref>PMID:38727003</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 8vci" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 19:58, 29 May 2024
SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop
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