7eep: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==== | ==Cyanophage Pam1 portal-adaptor complex== | ||
<StructureSection load='7eep' size='340' side='right'caption='[[7eep]]' scene=''> | <StructureSection load='7eep' size='340' side='right'caption='[[7eep]], [[Resolution|resolution]] 3.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7eep]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eep OCA], [https://pdbe.org/7eep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eep RCSB], [https://www.ebi.ac.uk/pdbsum/7eep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eep ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.75Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eep OCA], [https://pdbe.org/7eep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eep RCSB], [https://www.ebi.ac.uk/pdbsum/7eep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eep ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-A-long tail and an icosahedral capsid of 650 A in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a beta-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications. | |||
Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1.,Zhang JT, Yang F, Du K, Li WF, Chen Y, Jiang YL, Li Q, Zhou CZ Structure. 2022 Feb 3;30(2):240-251.e4. doi: 10.1016/j.str.2021.10.004. Epub 2021, Nov 1. PMID:34727518<ref>PMID:34727518</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7eep" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Unidentified]] | ||
[[Category: Jiang YL]] | |||
[[Category: Zhang JT]] | |||
[[Category: Zhou CZ]] | |||
Latest revision as of 05:43, 5 June 2024
Cyanophage Pam1 portal-adaptor complex
| ||||||||||||
