7sqk: Difference between revisions

Latest revision as of 05:52, 5 June 2024

Cryo-EM structure of the human augmin complex

Structural highlights

7sqk is a 8 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HAUS1_HUMAN Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Accurate segregation of chromosomes during mitosis depends on the correct assembly of the mitotic spindle, a bipolar structure composed mainly of microtubules. The augmin complex, or homologous to augmin subunits (HAUS) complex, is an eight-subunit protein complex required for building robust mitotic spindles in metazoa. Augmin increases microtubule density within the spindle by recruiting the gamma-tubulin ring complex (gamma-TuRC) to pre-existing microtubules and nucleating branching microtubules. Here, we elucidate the molecular architecture of augmin by single particle cryo-electron microscopy (cryo-EM), computational methods, and crosslinking mass spectrometry (CLMS). Augmin's highly flexible structure contains a V-shaped head and a filamentous tail, with the head existing in either extended or contracted conformational states. Our work highlights how cryo-EM, complemented by computational advances and CLMS, can elucidate the structure of a challenging protein complex and provides insights into the function of augmin in mediating microtubule branching nucleation.

Molecular architecture of the augmin complex.,Gabel CA, Li Z, DeMarco AG, Zhang Z, Yang J, Hall MC, Barford D, Chang L Nat Commun. 2022 Sep 16;13(1):5449. doi: 10.1038/s41467-022-33227-7. PMID:36114186^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Einarson MB, Cukierman E, Compton DA, Golemis EA. Human enhancer of invasion-cluster, a coiled-coil protein required for passage through mitosis. Mol Cell Biol. 2004 May;24(9):3957-71. PMID:15082789 doi:10.1128/MCB.24.9.3957-3971.2004
↑ Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6998-7003. doi:, 10.1073/pnas.0901587106. Epub 2009 Apr 14. PMID:19369198 doi:https://dx.doi.org/10.1073/pnas.0901587106
↑ Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L. HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity. Curr Biol. 2009 May 26;19(10):816-26. doi: 10.1016/j.cub.2009.04.033. Epub 2009, May 7. PMID:19427217 doi:https://dx.doi.org/10.1016/j.cub.2009.04.033
↑ Gabel CA, Li Z, DeMarco AG, Zhang Z, Yang J, Hall MC, Barford D, Chang L. Molecular architecture of the augmin complex. Nat Commun. 2022 Sep 16;13(1):5449. PMID:36114186 doi:10.1038/s41467-022-33227-7

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OCA

[1] Einarson MB, Cukierman E, Compton DA, Golemis EA. Human enhancer of invasion-cluster, a coiled-coil protein required for passage through mitosis. Mol Cell Biol. 2004 May;24(9):3957-71. PMID:15082789 doi:10.1128/MCB.24.9.3957-3971.2004

[2] Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6998-7003. doi:, 10.1073/pnas.0901587106. Epub 2009 Apr 14. PMID:19369198 doi:https://dx.doi.org/10.1073/pnas.0901587106

[3] Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L. HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity. Curr Biol. 2009 May 26;19(10):816-26. doi: 10.1016/j.cub.2009.04.033. Epub 2009, May 7. PMID:19427217 doi:https://dx.doi.org/10.1016/j.cub.2009.04.033

[4] Gabel CA, Li Z, DeMarco AG, Zhang Z, Yang J, Hall MC, Barford D, Chang L. Molecular architecture of the augmin complex. Nat Commun. 2022 Sep 16;13(1):5449. PMID:36114186 doi:10.1038/s41467-022-33227-7

[1]

[2]

[3]

[4]

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[7sqk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqk OCA], [https://pdbe.org/7sqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqk RCSB], [https://www.ebi.ac.uk/pdbsum/7sqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqk ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqk OCA], [https://pdbe.org/7sqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqk RCSB], [https://www.ebi.ac.uk/pdbsum/7sqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqk ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/HAUS2_HUMAN HAUS2_HUMAN]] Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.<ref>PMID:19369198</ref> <ref>PMID:19427217</ref>
+[https://www.uniprot.org/uniprot/HAUS1_HUMAN HAUS1_HUMAN] Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.<ref>PMID:15082789</ref> <ref>PMID:19369198</ref> <ref>PMID:19427217</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Accurate segregation of chromosomes during mitosis depends on the correct assembly of the mitotic spindle, a bipolar structure composed mainly of microtubules. The augmin complex, or homologous to augmin subunits (HAUS) complex, is an eight-subunit protein complex required for building robust mitotic spindles in metazoa. Augmin increases microtubule density within the spindle by recruiting the gamma-tubulin ring complex (gamma-TuRC) to pre-existing microtubules and nucleating branching microtubules. Here, we elucidate the molecular architecture of augmin by single particle cryo-electron microscopy (cryo-EM), computational methods, and crosslinking mass spectrometry (CLMS). Augmin's highly flexible structure contains a V-shaped head and a filamentous tail, with the head existing in either extended or contracted conformational states. Our work highlights how cryo-EM, complemented by computational advances and CLMS, can elucidate the structure of a challenging protein complex and provides insights into the function of augmin in mediating microtubule branching nucleation.
+Molecular architecture of the augmin complex.,Gabel CA, Li Z, DeMarco AG, Zhang Z, Yang J, Hall MC, Barford D, Chang L Nat Commun. 2022 Sep 16;13(1):5449. doi: 10.1038/s41467-022-33227-7. PMID:36114186<ref>PMID:36114186</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7sqk" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

7sqk: Difference between revisions

Latest revision as of 05:52, 5 June 2024

Cryo-EM structure of the human augmin complex

Structural highlights

Function

Publication Abstract from PubMed

References

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