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==48-nm repeat of the human respiratory doublet microtubule==
==48-nm repeat of the human respiratory doublet microtubule==
<StructureSection load='7ung' size='340' side='right'caption='[[7ung]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<SX load='7ung' size='340' side='right' viewer='molstar' caption='[[7ung]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7ung]] is a 325 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNG FirstGlance]. <br>
<table><tr><td colspan='2'>[[7ung]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ung FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ung OCA], [https://pdbe.org/7ung PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ung RCSB], [https://www.ebi.ac.uk/pdbsum/7ung PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ung ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ung FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ung OCA], [https://pdbe.org/7ung PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ung RCSB], [https://www.ebi.ac.uk/pdbsum/7ung PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ung ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/TEKT1_HUMAN TEKT1_HUMAN] Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.[UniProtKB:Q32KZ9]
[https://www.uniprot.org/uniprot/CP141_HUMAN CP141_HUMAN] Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.<ref>PMID:36191189</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients.
 
SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.,Gui M, Croft JT, Zabeo D, Acharya V, Kollman JM, Burgoyne T, Hoog JL, Brown A Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2207605119. doi: , 10.1073/pnas.2207605119. Epub 2022 Oct 3. PMID:36191189<ref>PMID:36191189</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ung" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
Retrieved from "https://proteopedia.org/7ung"