|
|
| Line 3: |
Line 3: |
| <StructureSection load='6txt' size='340' side='right'caption='[[6txt]]' scene=''> | | <StructureSection load='6txt' size='340' side='right'caption='[[6txt]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6txt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis Streptococcus sanguinis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TXT FirstGlance]. <br> | | <table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TXT FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6txt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6txt OCA], [https://pdbe.org/6txt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6txt RCSB], [https://www.ebi.ac.uk/pdbsum/6txt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6txt ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6txt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6txt OCA], [https://pdbe.org/6txt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6txt RCSB], [https://www.ebi.ac.uk/pdbsum/6txt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6txt ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
| |
| [https://www.uniprot.org/uniprot/A0A0B7GIC8_STRSA A0A0B7GIC8_STRSA]
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major monoderm class of Firmicutes. This also revealed that ComGC displays extensive sequence conservation, defining a monophyletic group among type IV pilins. We further report ComGC solution structures from two naturally competent human pathogens, Streptococcus sanguinis (ComGCSS) and Streptococcus pneumoniae (ComGCSP), revealing that this pilin displays extensive structural conservation. Strikingly, ComGCSS and ComGCSP exhibit a novel type IV pilin fold that is purely helical. Results from homology modelling analyses suggest that ComGC unusual structure is compatible with helical filament assembly. Because ComGC displays such a widespread distribution, these results have implications for hundreds of monoderm species.
| |
|
| |
| The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold.,Sheppard D, Berry JL, Denise R, Rocha EPC, Matthews S, Pelicic V J Biol Chem. 2020 Apr 9. pii: RA120.013316. doi: 10.1074/jbc.RA120.013316. PMID:32273343<ref>PMID:32273343</ref>
| |
|
| |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6txt" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Streptococcus sanguinis]]
| |
| [[Category: Berry JL]] | | [[Category: Berry JL]] |
| [[Category: Matthews SJ]] | | [[Category: Matthews SJ]] |
| [[Category: Pelicic V]] | | [[Category: Pelicic V]] |
| [[Category: Sheppard D]] | | [[Category: Sheppard D]] |
