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| <StructureSection load='7aed' size='340' side='right'caption='[[7aed]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='7aed' size='340' side='right'caption='[[7aed]], [[Resolution|resolution]] 1.75Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7aed]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AED FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AED FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.753Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aed OCA], [https://pdbe.org/7aed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aed RCSB], [https://www.ebi.ac.uk/pdbsum/7aed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aed ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aed OCA], [https://pdbe.org/7aed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aed RCSB], [https://www.ebi.ac.uk/pdbsum/7aed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aed ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
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| Multidrug-resistant bacteria pose serious problems in hospital-acquired infections (HAIs). Most antibiotic resistance genes are acquired via conjugative gene transfer, mediated by type 4 secretion systems (T4SS). Although most multidrug-resistant bacteria responsible for HAIs are of Gram-positive origin, with enterococci being major contributors, mostly Gram-negative T4SSs have been characterized. Here, we describe the structure and organization of PrgL, a core protein of the T4SS channel, encoded by the pCF10 plasmid from Enterococcus faecalis. The structure of PrgL displays similarity to VirB8 proteins of Gram-negative T4SSs. In vitro experiments show that the soluble domain alone is enough to drive both dimerization and dodecamerization, with a dimerization interface that differs from all other known VirB8-like proteins. In vivo experiments verify the importance of PrgL dimerization. Our findings provide insight into the molecular building blocks of Gram-positive T4SS, highlighting similarities but also unique features in PrgL compared to other VirB8-like proteins.
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| Structure of the enterococcal T4SS protein PrgL reveals unique dimerization interface in the VirB8 protein family.,Jager F, Lamy A, Sun WS, Guerini N, Berntsson RP Structure. 2022 Apr 6. pii: S0969-2126(22)00094-6. doi:, 10.1016/j.str.2022.03.013. PMID:35429437<ref>PMID:35429437</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7aed" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Berntsson, R P.A]] | | [[Category: Berntsson RPA]] |
| [[Category: Jaeger, F]] | | [[Category: Jaeger F]] |
| [[Category: Conjugation]]
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| [[Category: Membrane protein]]
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| [[Category: T4ss]]
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| [[Category: Virb8]]
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