8wb8: Difference between revisions
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==Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form== | |||
<StructureSection load='8wb8' size='340' side='right'caption='[[8wb8]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8wb8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ligilactobacillus_ruminis_ATCC_25644 Ligilactobacillus ruminis ATCC 25644]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WB8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wb8 OCA], [https://pdbe.org/8wb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wb8 RCSB], [https://www.ebi.ac.uk/pdbsum/8wb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wb8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/E7FRT5_9LACO E7FRT5_9LACO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sortase-dependent pili are long surface appendages that mediate attachment, colonization and biofilm formation in certain genera and species of Gram-positive bacteria. Ligilactobacillus ruminis is an autochthonous gut commensal that relies on sortase-dependent LrpCBA pili for host adherence and persistence. X-ray crystal structure snapshots of the backbone pilin LrpA were captured in two atypical bent conformations leading to a zigzag morphology in the LrpCBA pilus structure. Small-angle X-ray scattering and structural analysis revealed that LrpA also adopts the typical linear conformation, resulting in an elongated pilus morphology. Various conformational analyses and biophysical experiments helped to demonstrate that a hinge region located at the end of the flexible N-terminal domain of LrpA facilitates a new closure-and-twist motion for assembling dynamic pili during the assembly process and host attachment. Further, the incongruent combination of flexible domain-driven conformational dynamics and rigid isopeptide bond-driven stability observed in the LrpCBA pilus might also extend to the sortase-dependent pili of other bacteria colonizing a host. | |||
The crystal structure of the N-terminal domain of the backbone pilin LrpA reveals a new closure-and-twist motion for assembling dynamic pili in Ligilactobacillus ruminis.,Prajapati A, Palva A, von Ossowski I, Krishnan V Acta Crystallogr D Struct Biol. 2024 Jul 1;80(Pt 7):474-492. doi: , 10.1107/S2059798324005114. Epub 2024 Jun 27. PMID:38935340<ref>PMID:38935340</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8wb8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Ligilactobacillus ruminis ATCC 25644]] | |||
[[Category: Krishnan V]] | |||
[[Category: Palva A]] | |||
[[Category: Prajapati A]] | |||
[[Category: Von Ossowski I]] | |||
Latest revision as of 08:46, 14 July 2024
Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form
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