8x5v: Difference between revisions

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'''Unreleased structure'''


The entry 8x5v is ON HOLD  until Paper Publication
==BlCas9-sgRNA-target DNA complex==
<StructureSection load='8x5v' size='340' side='right'caption='[[8x5v]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8x5v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_laterosporus Brevibacillus laterosporus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8X5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8X5V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8x5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8x5v OCA], [https://pdbe.org/8x5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8x5v RCSB], [https://www.ebi.ac.uk/pdbsum/8x5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8x5v ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets complementary to an RNA guide, and is widely used as a powerful genome-editing tool. Here, we report the crystal structure of Brevibacillus laterosporus Cas9 (BlCas9, also known as BlatCas9), in complex with a guide RNA and its target DNA at 2.4-A resolution. The structure reveals that the BlCas9 guide RNA adopts an unexpected architecture containing a triple-helix, which is specifically recognized by BlCas9, and that BlCas9 recognizes a unique N(4)CNDN protospacer adjacent motif through base-specific interactions on both the target and non-target DNA strands. Based on the structure, we rationally engineered a BlCas9 variant that exhibits enhanced genome- and base-editing activities with an expanded target scope in human cells. This approach may further improve the performance of the enhanced BlCas9 variant to generate useful genome-editing tools that require only a single C PAM nucleotide and can be packaged into a single AAV vector for in vivo gene therapy.


Authors:  
Structure and engineering of Brevibacillus laterosporus Cas9.,Nakane T, Nakagawa R, Ishiguro S, Okazaki S, Mori H, Shuto Y, Yamashita K, Yachie N, Nishimasu H, Nureki O Commun Biol. 2024 Jul 3;7(1):803. doi: 10.1038/s42003-024-06422-z. PMID:38961195<ref>PMID:38961195</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 8x5v" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Brevibacillus laterosporus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Nakagawa R]]
[[Category: Nakane T]]
[[Category: Nishimasu H]]
[[Category: Nureki O]]
[[Category: Yamashita K]]
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