7swu: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
Line 3: Line 3:
<StructureSection load='7swu' size='340' side='right'caption='[[7swu]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
<StructureSection load='7swu' size='340' side='right'caption='[[7swu]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7swu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Stylophora_pistillata Stylophora pistillata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWU FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.444&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.444&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIV:2-[2-[(1~{S})-1,5-bis(azanyl)pentyl]-4-[(4-hydroxyphenyl)methyl]-5-oxidanylidene-imidazol-1-yl]ethanamide'>CIV</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIV:2-[2-[(1~{S})-1,5-bis(azanyl)pentyl]-4-[(4-hydroxyphenyl)methyl]-5-oxidanylidene-imidazol-1-yl]ethanamide'>CIV</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swu OCA], [https://pdbe.org/7swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swu RCSB], [https://www.ebi.ac.uk/pdbsum/7swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swu OCA], [https://pdbe.org/7swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swu RCSB], [https://www.ebi.ac.uk/pdbsum/7swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swu ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8CLX6_STYPI A8CLX6_STYPI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Anthozoan chromoproteins are highly pigmented, diversely coloured and readily produced in recombinant expression systems. While they are a versatile and powerful building block in synthetic biology for applications such as biosensor development, they are not widely used in comparison to the related fluorescent proteins, partly due to a lack of structural characterization to aid protein engineering. Here, high-resolution X-ray crystal structures of four open-source chromoproteins, gfasPurple, amilCP, spisPink and eforRed, are presented. These proteins are dimers in solution, and mutation at the conserved dimer interface leads to loss of visible colour development in gfasPurple. The chromophores are trans and noncoplanar in gfasPurple, amilCP and spisPink, while that in eforRed is cis and noncoplanar, and also emits fluorescence. Like other characterized chromoproteins, gfasPurple, amilCP and eforRed contain an sp(2)-hybridized N-acylimine in the peptide bond preceding the chromophore, while spisPink is unusual and demonstrates a true sp(3)-hybridized trans-peptide bond at this position. It was found that point mutations at the chromophore-binding site in gfasPurple that substitute similar amino acids to those in amilCP and spisPink generate similar colours. These features and observations have implications for the utility of these chromoproteins in protein engineering and synthetic biology applications.
Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.,Ahmed FH, Caputo AT, French NG, Peat TS, Whitfield J, Warden AC, Newman J, Scott C Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):599-612. doi:, 10.1107/S2059798322002625. Epub 2022 Apr 8. PMID:35503208<ref>PMID:35503208</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7swu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Stylophora pistillata]]
[[Category: Ahmed H]]
[[Category: Ahmed H]]
[[Category: Caputo AT]]
[[Category: Caputo AT]]
[[Category: Newman J]]
[[Category: Newman J]]
[[Category: Scott C]]
[[Category: Scott C]]

Latest revision as of 09:02, 14 July 2024

Crystal structure of the chromoprotein spisPINK

7swu, resolution 1.44Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=7swu&oldid=4190731"