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==Cryo EM structure of bison NHA2 in nano disc structure==
==Structure of ion transporter==
<StructureSection load='7p1k' size='340' side='right'caption='[[7p1k]], [[Resolution|resolution]] 3.64&Aring;' scene=''>
<StructureSection load='7p1k' size='340' side='right'caption='[[7p1k]], [[Resolution|resolution]] 3.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7p1k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P1K FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P1K FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T7X:PHOSPHATIDYLINOSITOL'>T7X</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.64&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T7X:PHOSPHATIDYLINOSITOL'>T7X</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p1k OCA], [https://pdbe.org/7p1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p1k RCSB], [https://www.ebi.ac.uk/pdbsum/7p1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p1k ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p1k OCA], [https://pdbe.org/7p1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p1k RCSB], [https://www.ebi.ac.uk/pdbsum/7p1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p1k ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Na(+)/H(+) exchanger SLC9B2, also known as NHA2, correlates with the long-sought-after Na(+)/Li(+) exchanger linked to the pathogenesis of diabetes mellitus and essential hypertension in humans. Despite the functional importance of NHA2, structural information and the molecular basis for its ion-exchange mechanism have been lacking. Here we report the cryo-EM structures of bison NHA2 in detergent and in nanodiscs, at 3.0 and 3.5 A resolution, respectively. The bison NHA2 structure, together with solid-state membrane-based electrophysiology, establishes the molecular basis for electroneutral ion exchange. NHA2 consists of 14 transmembrane (TM) segments, rather than the 13 TMs previously observed in mammalian Na(+)/H(+) exchangers (NHEs) and related bacterial antiporters. The additional N-terminal helix in NHA2 forms a unique homodimer interface with a large intracellular gap between the protomers, which closes in the presence of phosphoinositol lipids. We propose that the additional N-terminal helix has evolved as a lipid-mediated remodeling switch for the regulation of NHA2 activity.
Structure, mechanism and lipid-mediated remodeling of the mammalian Na(+)/H(+) exchanger NHA2.,Matsuoka R, Fudim R, Jung S, Zhang C, Bazzone A, Chatzikyriakidou Y, Robinson CV, Nomura N, Iwata S, Landreh M, Orellana L, Beckstein O, Drew D Nat Struct Mol Biol. 2022 Feb;29(2):108-120. doi: 10.1038/s41594-022-00738-2., Epub 2022 Feb 16. PMID:35173351<ref>PMID:35173351</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7p1k" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Drew, D]]
[[Category: Drew D]]
[[Category: Fudim, R]]
[[Category: Fudim R]]
[[Category: Jung, S]]
[[Category: Jung S]]
[[Category: Matsuoka, R]]
[[Category: Matsuoka R]]
[[Category: Membrane protein sodium proton transporter]]
[[Category: Transport protein]]

Latest revision as of 12:29, 17 July 2024

Structure of ion transporter

7p1k, resolution 3.64Å

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