8brj

Escherichia coli anaerobic fatty acid beta oxidation trifunctional enzyme (anEcTFE) trimeric complex

spinqualitylabelsall models 8brj, resolution 4.08Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8brj is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.08Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FADI_ECOLI Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids. Publication Abstract from PubMed Facultative anaerobic bacteria such as Escherichia coli have two alpha(2)beta(2) heterotetrameric trifunctional enzymes (TFE), catalyzing the last three steps of the beta-oxidation cycle: soluble aerobic TFE (EcTFE) and membrane-associated anaerobic TFE (anEcTFE), closely related to the human mitochondrial TFE (HsTFE). The cryo-EM structure of anEcTFE and crystal structures of anEcTFE-alpha show that the overall assembly of anEcTFE and HsTFE is similar. However, their membrane-binding properties differ considerably. The shorter A5-H7 and H8 regions of anEcTFE-alpha result in weaker alpha-beta as well as alpha-membrane interactions, respectively. The protruding H-H region of anEcTFE-beta is therefore more critical for membrane-association. Mutational studies also show that this region is important for the stability of the anEcTFE-beta dimer and anEcTFE heterotetramer. The fatty acyl tail binding tunnel of the anEcTFE-alpha hydratase domain, as in HsTFE-alpha, is wider than in EcTFE-alpha, accommodating longer fatty acyl tails, in good agreement with their respective substrate specificities. Structural basis for different membrane-binding properties of E. coli anaerobic and human mitochondrial beta-oxidation trifunctional enzymes.,Sah-Teli SK, Pinkas M, Hynonen MJ, Butcher SJ, Wierenga RK, Novacek J, Venkatesan R Structure. 2023 Jul 6;31(7):812-825.e6. doi: 10.1016/j.str.2023.04.011. Epub 2023 , May 15. PMID:37192613 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA