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==Solid-state NMR structure of the D-Arg4,L10-teixobactin - Lipid II complex in lipid bilayers.==
==Solid-state NMR structure of the D-Arg4,L10-teixobactin - Lipid II complex in lipid bilayers==
<StructureSection load='6yfy' size='340' side='right'caption='[[6yfy]]' scene=''>
<StructureSection load='6yfy' size='340' side='right'caption='[[6yfy]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6yfy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Eleftheria_terrae Eleftheria terrae] and [https://en.wikipedia.org/wiki/Staphylococcus_simulans Staphylococcus simulans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YFY FirstGlance]. <br>
<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YFY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=28J:D-ALLOISOLEUCINE'>28J</scene>, <scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=DTH:D-THREONINE'>DTH</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P1W:3-methylbut-2-en-1-ol'>P1W</scene>, <scene name='pdbligand=PRD_002268:Lipid+II'>PRD_002268</scene>, <scene name='pdbligand=ZAE:N-METHYL-D-PHENYLALANINE'>ZAE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=28J:D-ALLOISOLEUCINE'>28J</scene>, <scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=DTH:D-THREONINE'>DTH</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P1W:3-methylbut-2-en-1-ol'>P1W</scene>, <scene name='pdbligand=ZAE:N-METHYL-D-PHENYLALANINE'>ZAE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfy OCA], [https://pdbe.org/6yfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yfy RCSB], [https://www.ebi.ac.uk/pdbsum/6yfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfy OCA], [https://pdbe.org/6yfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yfy RCSB], [https://www.ebi.ac.uk/pdbsum/6yfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfy ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The natural antibiotic teixobactin kills pathogenic bacteria without detectable resistance. The difficult synthesis and unfavourable solubility of teixobactin require modifications, yet insufficient knowledge on its binding mode impedes the hunt for superior analogues. Thus far, teixobactins are assumed to kill bacteria by binding to cognate cell wall precursors (Lipid II and III). Here we present the binding mode of teixobactins in cellular membranes using solid-state NMR, microscopy, and affinity assays. We solve the structure of the complex formed by an improved teixobactin-analogue and Lipid II and reveal how teixobactins recognize a broad spectrum of targets. Unexpectedly, we find that teixobactins only weakly bind to Lipid II in cellular membranes, implying the direct interaction with cell wall precursors is not the sole killing mechanism. Our data suggest an additional mechanism affords the excellent activity of teixobactins, which can block the cell wall biosynthesis by capturing precursors in massive clusters on membranes.
Mode of action of teixobactins in cellular membranes.,Shukla R, Medeiros-Silva J, Parmar A, Vermeulen BJA, Das S, Paioni AL, Jekhmane S, Lorent J, Bonvin AMJJ, Baldus M, Lelli M, Veldhuizen EJA, Breukink E, Singh I, Weingarth M Nat Commun. 2020 Jun 5;11(1):2848. doi: 10.1038/s41467-020-16600-2. PMID:32503964<ref>PMID:32503964</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6yfy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Eleftheria terrae]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Staphylococcus simulans]]
[[Category: Shukla R]]
[[Category: Shukla R]]
[[Category: Weingarth MH]]
[[Category: Weingarth MH]]

Latest revision as of 05:47, 7 August 2024

Solid-state NMR structure of the D-Arg4,L10-teixobactin - Lipid II complex in lipid bilayers

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