4g2t: Difference between revisions
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==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate== | ==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate== | ||
<StructureSection load='4g2t' size='340' side='right'caption='[[4g2t]], [[Resolution|resolution]] 2. | <StructureSection load='4g2t' size='340' side='right'caption='[[4g2t]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SF2575 Streptomyces sp. SF2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G2T FirstGlance]. <br> | <table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SF2575 Streptomyces sp. SF2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G2T FirstGlance]. <br> | ||
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/D6MSX4_9ACTN D6MSX4_9ACTN] | [https://www.uniprot.org/uniprot/D6MSX4_9ACTN D6MSX4_9ACTN] | ||
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== Publication Abstract from PubMed == | |||
The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc. | |||
Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584<ref>PMID:23526584</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 4g2t" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
