8rnp: Difference between revisions
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==Unspecific peroxygenase from Marasmius wettsteinii (MweUPO-1) in complex with R-limonene== | |||
<StructureSection load='8rnp' size='340' side='right'caption='[[8rnp]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8rnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marasmius_wettsteinii Marasmius wettsteinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RNP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9IR:(4~{R})-1-methyl-4-prop-1-en-2-yl-cyclohexene'>9IR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rnp OCA], [https://pdbe.org/8rnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rnp RCSB], [https://www.ebi.ac.uk/pdbsum/8rnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rnp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fungal unspecific peroxygenases (UPOs) are gaining momentum in synthetic chemistry. Of special interest is the UPO from Marasmius rotula (MroUPO), which shows an exclusive repertoire of oxyfunctionalizations, including the terminal hydroxylation of alkanes, the alpha-oxidation of fatty acids and the C-C cleavage of corticosteroids. However, the lack of heterologous expression systems to perform directed evolution has impeded its engineering for practical applications. Here, we introduce a close ortholog of MroUPO, a UPO gene from Marasmius wettsteinii (MweUPO-1), that has a similar reaction profile to MroUPO and for which we have set up a directed evolution platform based on tandem-yeast expression. Recombinant MweUPO-1 was produced at high titers in the bioreactor (0.7 g/L) and characterized at the biochemical and atomic levels. The conjunction of soaking crystallographic experiments at a resolution up to 1.6 A together with the analysis of reaction patterns sheds light on the substrate preferences of this promiscuous biocatalyst. | |||
Structural Insights and Reaction Profile of a New Unspecific Peroxygenase from Marasmius wettsteinii Produced in a Tandem-Yeast Expression System.,Sanchez-Moreno I, Fernandez-Garcia A, Mateljak I, Gomez de Santos P, Hofrichter M, Kellner H, Sanz-Aparicio J, Alcalde M ACS Chem Biol. 2024 Oct 5. doi: 10.1021/acschembio.4c00504. PMID:39367827<ref>PMID:39367827</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Fernandez-Garcia | <div class="pdbe-citations 8rnp" style="background-color:#fffaf0;"></div> | ||
[[Category: Sanz-Aparicio | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Marasmius wettsteinii]] | |||
[[Category: Fernandez-Garcia A]] | |||
[[Category: Sanz-Aparicio J]] | |||
Latest revision as of 04:12, 17 October 2024
Unspecific peroxygenase from Marasmius wettsteinii (MweUPO-1) in complex with R-limonene
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