8y82: Difference between revisions
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==Cryo-EM structure of the tetrameric SPARSA gRNA-ssDNA-NAD+ complex== | |||
<StructureSection load='8y82' size='340' side='right'caption='[[8y82]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8y82]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_'BL21-Gold(DE3)pLysS_AG' Escherichia coli 'BL21-Gold(DE3)pLysS AG'] and [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y82 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y82 OCA], [https://pdbe.org/8y82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y82 RCSB], [https://www.ebi.ac.uk/pdbsum/8y82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y82 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q74DF6_GEOSL Q74DF6_GEOSL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Eukaryotic Argonaute proteins (eAgos) utilize short nucleic acid guides to target complementary sequences for RNA silencing, while prokaryotic Agos (pAgos) provide immunity against invading plasmids or bacteriophages. The Sir2-domain associated short pAgo (SPARSA) immune system defends against invaders by depleting NAD(+) and triggering cell death. However, the molecular mechanism underlying SPARSA activation remains unknown. Here, we present cryo-EM structures of inactive monomeric, active tetrameric and active NAD(+)-bound tetrameric SPARSA complexes, elucidating mechanisms underlying SPARSA assembly, guide RNA preference, target ssDNA-triggered SPARSA tetramerization, and tetrameric-dependent NADase activation. Short pAgos form heterodimers with Sir2-APAZ, favoring short guide RNA with a 5'-AU from ColE-like plasmids. RNA-guided recognition of the target ssDNA triggers SPARSA tetramerization via pAgo- and Sir2-mediated interactions. The resulting tetrameric Sir2 rearrangement aligns catalytic residue H186 for NAD(+) hydrolysis. These insights advance our understanding of Sir2-domain associated pAgos immune systems and should facilitate the development of a short pAgo-associated biotechnological toolbox. | |||
Tetramerization-dependent activation of the Sir2-associated short prokaryotic Argonaute immune system.,Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N Nat Commun. 2024 Oct 4;15(1):8610. doi: 10.1038/s41467-024-52910-5. PMID:39366953<ref>PMID:39366953</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8y82" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Geobacter sulfurreducens]] | |||
[[Category: Large Structures]] | |||
[[Category: Cui N]] | |||
[[Category: Jia N]] | |||
[[Category: Wei XY]] | |||
[[Category: Zhang JT]] | |||
