3tw5

Crystal structure of the GP42 transglutaminase from Phytophthora sojae

spinqualitylabelsall models 3tw5, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3tw5 is a 2 chain structure with sequence from Phytophthora sojae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.95Å Ligands: CXS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q01928_PHYSO Publication Abstract from PubMed Transglutaminases (TGases) are ubiquitous enzymes that catalyze selective crosslinking between protein-bound glutamine and lysine residues; the resulting isopeptide bond confers high resistance to proteolysis. Phytophthora sojae, a pathogen of soybean, secretes a Ca(2+)-dependent TGase (GP42) that is activating defense responses in both host and non-host plants. A GP42 fragment of 13 amino acids, termed Pep-13, was shown to be absolutely indispensable for both TGase and elicitor activity. GP42 does not share significant primary sequence similarity with known TGases from mammals or bacteria. This suggests that GP42 has evolved novel structural and catalytic features to support enzymatic activity. We have solved the crystal structure of the catalytically inactive point mutant GP42 (C290S) at 2.95 A resolution and identified residues involved in catalysis by mutational analysis. The protein comprises three domains that assemble into an elongated structure. Although GP42 has no structural homolog, its core region displays significant similarity to the catalytic core of the Mac-1 cysteine protease from Group A Streptococcus, a member of the papain-like superfamily of cysteine proteases. Proteins that are taxonomically related to GP42 are only present in plant pathogenic oomycetes belonging to the order of the Peronosporales (e.g Phytophthora, Hyaloperonospora and Pythium spp) and in marine Vibrio bacteria. This suggests that a lateral gene transfer event may have occurred between bacteria and oomycetes. Our results offer a basis to design and use highly specific inhibitors of the GP42-like TGase family that may impair the growth of important oomycete and bacterial pathogens. Structural and phylogenetic analyses of the GP42 transglutaminase from Phytophthora sojae reveal an evolutionary relationship between oomycetes and marine Vibrio bacteria.,Reiss K, Kirchner E, Gijzen M, Zocher G, Loeffelhardt B, Nuernberger T, Stehle T, Brunner F J Biol Chem. 2011 Oct 12. PMID:21994936 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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