5w4d: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w4d OCA], [https://pdbe.org/5w4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w4d RCSB], [https://www.ebi.ac.uk/pdbsum/5w4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w4d ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w4d OCA], [https://pdbe.org/5w4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w4d RCSB], [https://www.ebi.ac.uk/pdbsum/5w4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w4d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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== Publication Abstract from PubMed == | |||
Cytoplasmic RNA-protein (RNP) granules have diverse biophysical properties, from liquid to solid, and play enigmatic roles in RNA metabolism. Nematode P granules are paradigmatic liquid droplet granules and central to germ cell development. Here we analyze a key P granule scaffolding protein, PGL-1, to investigate the functional relationship between P granule assembly and function. Using a protein-RNA tethering assay, we find that reporter mRNA expression is repressed when recruited to PGL-1. We determine the crystal structure of the PGL-1 N-terminal region to 1.5 A, discover its dimerization, and identify key residues at the dimer interface. Mutations of those interface residues prevent P granule assembly in vivo, de-repress PGL-1 tethered mRNA, and reduce fertility. Therefore, PGL-1 dimerization lies at the heart of both P granule assembly and function. Finally, we identify the P granule-associated Argonaute WAGO-1 as crucial for repression of PGL-1 tethered mRNA. We conclude that P granule function requires both assembly and localized regulators. | |||
C. elegans germ granules require both assembly and localized regulators for mRNA repression.,Aoki ST, Lynch TR, Crittenden SL, Bingman CA, Wickens M, Kimble J Nat Commun. 2021 Feb 12;12(1):996. doi: 10.1038/s41467-021-21278-1. PMID:33579952<ref>PMID:33579952</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 5w4d" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | </StructureSection> | ||
Latest revision as of 07:42, 17 October 2024
C. japonica N-domain, Selenomethionine mutant
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