7ssl: Difference between revisions
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==== | ==Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III)== | ||
<StructureSection load='7ssl' size='340' side='right'caption='[[7ssl]]' scene=''> | <StructureSection load='7ssl' size='340' side='right'caption='[[7ssl]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ssl]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SSL FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ssl OCA], [https://pdbe.org/7ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ssl RCSB], [https://www.ebi.ac.uk/pdbsum/7ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ssl ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ssl OCA], [https://pdbe.org/7ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ssl RCSB], [https://www.ebi.ac.uk/pdbsum/7ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ssl ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
During translation, a conserved GTPase elongation factor-EF-G in bacteria or eEF2 in eukaryotes-translocates tRNA and mRNA through the ribosome. EF-G has been proposed to act as a flexible motor that propels tRNA and mRNA movement, as a rigid pawl that biases unidirectional translocation resulting from ribosome rearrangements, or by various combinations of motor- and pawl-like mechanisms. Using time-resolved cryo-EM, we visualized GTP-catalyzed translocation without inhibitors, capturing elusive structures of ribosome*EF-G intermediates at near-atomic resolution. Prior to translocation, EF-G binds near peptidyl-tRNA, while the rotated 30S subunit stabilizes the EF-G GTPase center. Reverse 30S rotation releases Pi and translocates peptidyl-tRNA and EF-G by ~20 A. An additional 4-A translocation initiates EF-G dissociation from a transient ribosome state with highly swiveled 30S head. The structures visualize how nearly rigid EF-G rectifies inherent and spontaneous ribosomal dynamics into tRNA-mRNA translocation, whereas GTP hydrolysis and Pi release drive EF-G dissociation. | |||
Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP.,Carbone CE, Loveland AB, Gamper HB Jr, Hou YM, Demo G, Korostelev AA Nat Commun. 2021 Dec 13;12(1):7236. doi: 10.1038/s41467-021-27415-0. PMID:34903725<ref>PMID:34903725</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ssl" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Elongation factor 3D structures|Elongation factor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Carbone CE]] | ||
[[Category: Korostelev AA]] | |||
Latest revision as of 09:11, 17 October 2024
Pre translocation intermediate with EF-G bound to GDP and Pi (Structure III)
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