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'''NMR STUDY OF Z-DNA AT PHYSIOLOGICAL SALT CONDITIONS, MINIMIZED AVERAGE STRUCTURE''' | '''NMR STUDY OF Z-DNA AT PHYSIOLOGICAL SALT CONDITIONS, MINIMIZED AVERAGE STRUCTURE''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TNE OCA]. | |||
==Reference== | ==Reference== | ||
Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions., Sugiyama H, Kawai K, Matsunaga A, Fujimoto K, Saito I, Robinson H, Wang AH, Nucleic Acids Res. 1996 Apr 1;24(7):1272-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8614630 8614630] | Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions., Sugiyama H, Kawai K, Matsunaga A, Fujimoto K, Saito I, Robinson H, Wang AH, Nucleic Acids Res. 1996 Apr 1;24(7):1272-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8614630 8614630] | ||
[[Category: Fujimoto, K.]] | [[Category: Fujimoto, K.]] | ||
[[Category: Kawai, K.]] | [[Category: Kawai, K.]] | ||
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[[Category: Sugiyama, H.]] | [[Category: Sugiyama, H.]] | ||
[[Category: Wang, A H.J.]] | [[Category: Wang, A H.J.]] | ||
[[Category: | [[Category: Z-dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:09:25 2008'' | |||
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Revision as of 07:09, 3 May 2008
NMR STUDY OF Z-DNA AT PHYSIOLOGICAL SALT CONDITIONS, MINIMIZED AVERAGE STRUCTURE
Overview
Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly stabilizes the Z conformation under low salt conditions. The hexamer d(CGC[M8G]CG)2 exhibits a CD spectrum characteristic of the Z conformation under physiological salt conditions. The NOE-restrained refinement unequivocally demonstrated that d(CGC[m8G]CG)2 adopts a Z structure with all guanines in the syn conformation. The refined NMR structure is very similar to the Z form crystal structure of d(CGCGCG)2, with a root mean square deviation of 0.6 between the two structures. The contribution of m8G to the stabilization of Z-DNA has been estimated from the mid-point NaCl concentrations for the B-Z transition of various m8G-containing oligomers. The presence of m8G in d(CGC[m8G]CG)2 stabilizes the Z conformation by at least deltaG = -0.8 kcal/mol relative to the unmodified hexamer. The Z conformation was further stabilized by increasing the number of m8Gs incorporated and destabilized by incorporating syn-A or syn-T, found respectively in the (A,T)-containing alternating and non-alternating pyrimidine-purine sequences. The results suggest that the chemically less reactive m8G base is a useful agent for studying molecular interactions of Z-DNA or other DNA structures that incorporate syn-G conformation.
About this Structure
Full crystallographic information is available from OCA.
Reference
Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions., Sugiyama H, Kawai K, Matsunaga A, Fujimoto K, Saito I, Robinson H, Wang AH, Nucleic Acids Res. 1996 Apr 1;24(7):1272-8. PMID:8614630 Page seeded by OCA on Sat May 3 10:09:25 2008
