5zhs: Difference between revisions

Latest revision as of 09:39, 23 October 2024

Crystal structure of OsD14 in complex with covalently bound KK052

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.49Å
Ligands:	KAT
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Strigolactones, a class of plant hormones with multiple functions, mediate plant-plant and plant-microorganism communications in the rhizosphere. In this study, we developed potent strigolactone antagonists, which covalently bind to the strigolactone receptor D14, by preparing an array of triazole urea compounds. Using yeast two-hybrid and rice-tillering assays, we identified a triazole urea compound KK094 as a potent inhibitor of strigolactone receptors. Liquid chromatography-tandem mass spectrometry analysis and X-ray crystallography revealed that KK094 was hydrolyzed by D14, and that a reaction product of this degradation covalently binds to the Ser residue of the catalytic triad of D14. Furthermore, we identified two triazole urea compounds KK052 and KK073, whose effects on D14-D53/D14-SLR1 complex formation were opposite due to the absence (KK052) or presence (KK073) of a trifluoromethyl group on their phenyl ring. These results demonstrate that triazole urea compounds are potentially powerful tools for agricultural application and may be useful for the elucidation of the complicated mechanism underlying strigolactone perception.

Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses.,Nakamura H, Hirabayashi K, Miyakawa T, Kikuzato K, Hu W, Xu Y, Jiang K, Takahashi I, Niiyama R, Dohmae N, Tanokura M, Asami T Mol Plant. 2019 Jan 7;12(1):44-58. doi: 10.1016/j.molp.2018.10.006. Epub 2018 Nov, 2. PMID:30391752^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nakamura H, Hirabayashi K, Miyakawa T, Kikuzato K, Hu W, Xu Y, Jiang K, Takahashi I, Niiyama R, Dohmae N, Tanokura M, Asami T. Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses. Mol Plant. 2019 Jan 7;12(1):44-58. PMID:30391752 doi:10.1016/j.molp.2018.10.006

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Nakamura H, Hirabayashi K, Miyakawa T, Kikuzato K, Hu W, Xu Y, Jiang K, Takahashi I, Niiyama R, Dohmae N, Tanokura M, Asami T. Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses. Mol Plant. 2019 Jan 7;12(1):44-58. PMID:30391752 doi:10.1016/j.molp.2018.10.006

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5zhs' size='340' side='right'caption='[[5zhs]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zhs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHS FirstGlance]. <br>
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHS FirstGlance]. <br>
 </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
 <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KAT:(4-phenylpiperazin-1-yl)(1H-1,2,3-triazol-1-yl)methanone'>KAT</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhs OCA], [https://pdbe.org/5zhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zhs RCSB], [https://www.ebi.ac.uk/pdbsum/5zhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhs ProSAT]</span></td></tr>
 </table>
-== Function ==
-[https://www.uniprot.org/uniprot/D14_ORYSJ D14_ORYSJ] Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.<ref>PMID:19542179</ref> <ref>PMID:23301669</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Oryza sativa Japonica Group]]
 [[Category: Hirabayashi K]]
 [[Category: Miyakawa T]]
 [[Category: Tanokura M]]

5zhs: Difference between revisions

Latest revision as of 09:39, 23 October 2024

Crystal structure of OsD14 in complex with covalently bound KK052

Structural highlights

Publication Abstract from PubMed

References

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