6gd5: Difference between revisions

Latest revision as of 09:58, 23 October 2024

The solution structure of the LptA-Thanatin complex

Structural highlights

6gd5 is a 2 chain structure with sequence from Escherichia coli K-12 and Podisus maculiventris. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LPTA_ECOLI Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

With the increasing resistance of many Gram-negative bacteria to existing classes of antibiotics, identifying new paradigms in antimicrobial discovery is an important research priority. Of special interest are the proteins required for the biogenesis of the asymmetric Gram-negative bacterial outer membrane (OM). Seven Lpt proteins (LptA to LptG) associate in most Gram-negative bacteria to form a macromolecular complex spanning the entire envelope, which transports lipopolysaccharide (LPS) molecules from their site of assembly at the inner membrane to the cell surface, powered by adenosine 5'-triphosphate hydrolysis in the cytoplasm. The periplasmic protein LptA comprises the protein bridge across the periplasm, which connects LptB2FGC at the inner membrane to LptD/E anchored in the OM. We show here that the naturally occurring, insect-derived antimicrobial peptide thanatin targets LptA and LptD in the network of periplasmic protein-protein interactions required to assemble the Lpt complex, leading to the inhibition of LPS transport and OM biogenesis in Escherichia coli.

Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli.,Vetterli SU, Zerbe K, Muller M, Urfer M, Mondal M, Wang SY, Moehle K, Zerbe O, Vitale A, Pessi G, Eberl L, Wollscheid B, Robinson JA Sci Adv. 2018 Nov 14;4(11):eaau2634. doi: 10.1126/sciadv.aau2634. eCollection, 2018 Nov. PMID:30443594^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014
↑ Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A. Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol. 2007 Jan;189(1):244-53. Epub 2006 Oct 20. PMID:17056748 doi:https://dx.doi.org/10.1128/JB.01126-06
↑ Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08
↑ Tran AX, Trent MS, Whitfield C. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem. 2008 Jul 18;283(29):20342-9. doi: 10.1074/jbc.M802503200. Epub 2008 , May 14. PMID:18480051 doi:https://dx.doi.org/10.1074/jbc.M802503200
↑ Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A. New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol. 2011 Mar;193(5):1042-53. doi: 10.1128/JB.01037-10. Epub 2010 Dec 17. PMID:21169485 doi:https://dx.doi.org/10.1128/JB.01037-10
↑ Vetterli SU, Zerbe K, Muller M, Urfer M, Mondal M, Wang SY, Moehle K, Zerbe O, Vitale A, Pessi G, Eberl L, Wollscheid B, Robinson JA. Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli. Sci Adv. 2018 Nov 14;4(11):eaau2634. doi: 10.1126/sciadv.aau2634. eCollection, 2018 Nov. PMID:30443594 doi:https://dx.doi.org/10.1126/sciadv.aau2634

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OCA

[1] Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014

[2] Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A. Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol. 2007 Jan;189(1):244-53. Epub 2006 Oct 20. PMID:17056748 doi:https://dx.doi.org/10.1128/JB.01126-06

[3] Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08

[4] Tran AX, Trent MS, Whitfield C. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem. 2008 Jul 18;283(29):20342-9. doi: 10.1074/jbc.M802503200. Epub 2008 , May 14. PMID:18480051 doi:https://dx.doi.org/10.1074/jbc.M802503200

[5] Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A. New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol. 2011 Mar;193(5):1042-53. doi: 10.1128/JB.01037-10. Epub 2010 Dec 17. PMID:21169485 doi:https://dx.doi.org/10.1128/JB.01037-10

[6] Vetterli SU, Zerbe K, Muller M, Urfer M, Mondal M, Wang SY, Moehle K, Zerbe O, Vitale A, Pessi G, Eberl L, Wollscheid B, Robinson JA. Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli. Sci Adv. 2018 Nov 14;4(11):eaau2634. doi: 10.1126/sciadv.aau2634. eCollection, 2018 Nov. PMID:30443594 doi:https://dx.doi.org/10.1126/sciadv.aau2634

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@@ Line 1: / Line 1: @@
 ==The solution structure of the LptA-Thanatin complex==
-<StructureSection load='6gd5' size='340' side='right'caption='[[6gd5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='6gd5' size='340' side='right'caption='[[6gd5]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6gd5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Podma Podma]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GD5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GD5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6gd5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Podisus_maculiventris Podisus maculiventris]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GD5 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lptA, yhbN, b3200, JW3167 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gd5 OCA], [http://pdbe.org/6gd5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gd5 RCSB], [http://www.ebi.ac.uk/pdbsum/6gd5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gd5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gd5 OCA], [https://pdbe.org/6gd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gd5 RCSB], [https://www.ebi.ac.uk/pdbsum/6gd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gd5 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LPTA_ECOLI LPTA_ECOLI]] Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]<ref>PMID:16765569</ref> <ref>PMID:17056748</ref> <ref>PMID:18424520</ref> <ref>PMID:18480051</ref> <ref>PMID:21169485</ref>  [[http://www.uniprot.org/uniprot/THAN_PODMA THAN_PODMA]] Insect defense peptide with a broad spectrum of activity against Gram-positive and Gram-negative bacteria and fungi. No activity against S.aureus. Stops respiration in bacteria but does not permeabilize their inner membranes.
+[https://www.uniprot.org/uniprot/LPTA_ECOLI LPTA_ECOLI] Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]<ref>PMID:16765569</ref> <ref>PMID:17056748</ref> <ref>PMID:18424520</ref> <ref>PMID:18480051</ref> <ref>PMID:21169485</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Podma]]
+[[Category: Podisus maculiventris]]
-[[Category: Moehle, K]]
+[[Category: Moehle K]]
-[[Category: Zerbe, O]]
+[[Category: Zerbe O]]
-[[Category: Antibiotic]]
-[[Category: Lps biosynthesis inhibitor complex nmr]]

6gd5: Difference between revisions

Latest revision as of 09:58, 23 October 2024

The solution structure of the LptA-Thanatin complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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