6r1h: Difference between revisions

Latest revision as of 10:24, 23 October 2024

Crystal structure of the LRR ectodomain of the receptor kinase SOBIR1 from Arabidopsis thaliana.

Structural highlights

6r1h is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.55Å
Ligands:	CL, EDO, GOL, NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SBIR1_ARATH Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death (PubMed:19616764). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity (PubMed:27251392). Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission (PubMed:20081191).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Plant-unique membrane receptor kinases with leucine-rich repeat (LRR) extracellular domains are key regulators of development and immune responses. Here, the 1.55 A resolution crystal structure of the immune receptor kinase SOBIR1 from Arabidopsis is presented. The ectodomain structure reveals the presence of five LRRs sandwiched between noncanonical capping domains. The disulfide-bond-stabilized N-terminal cap harbours an unusual beta-hairpin structure. The C-terminal cap features a highly positively charged linear motif which was found to be largely disordered in this structure. Size-exclusion chromatography and right-angle light-scattering experiments suggest that SOBIR1 is a monomer in solution. The protruding beta-hairpin, a set of highly conserved basic residues at the inner surface of the SOBIR LRR domain and the presence of a genetic missense allele in LRR2 together suggest that the SOBIR1 ectodomain may mediate protein-protein interaction in plant immune signalling.

Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1.,Hohmann U, Hothorn M Acta Crystallogr D Struct Biol. 2019 May 1;75(Pt 5):488-497. doi:, 10.1107/S2059798319005291. Epub 2019 Apr 29. PMID:31063151^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gao M, Wang X, Wang D, Xu F, Ding X, Zhang Z, Bi D, Cheng YT, Chen S, Li X, Zhang Y. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 2009 Jul 23;6(1):34-44. doi: 10.1016/j.chom.2009.05.019. PMID:19616764 doi:https://dx.doi.org/10.1016/j.chom.2009.05.019
↑ Leslie ME, Lewis MW, Youn JY, Daniels MJ, Liljegren SJ. The EVERSHED receptor-like kinase modulates floral organ shedding in Arabidopsis. Development. 2010 Feb;137(3):467-76. doi: 10.1242/dev.041335. PMID:20081191 doi:https://dx.doi.org/10.1242/dev.041335
↑ Albert I, Bohm H, Albert M, Feiler CE, Imkampe J, Wallmeroth N, Brancato C, Raaymakers TM, Oome S, Zhang H, Krol E, Grefen C, Gust AA, Chai J, Hedrich R, Van den Ackerveken G, Nurnberger T. An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nat Plants. 2015 Oct 5;1:15140. doi: 10.1038/nplants.2015.140. PMID:27251392 doi:https://dx.doi.org/10.1038/nplants.2015.140
↑ Hohmann U, Hothorn M. Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1. Acta Crystallogr D Struct Biol. 2019 May 1;75(Pt 5):488-497. doi:, 10.1107/S2059798319005291. Epub 2019 Apr 29. PMID:31063151 doi:https://dx.doi.org/10.1107/S2059798319005291

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Gao M, Wang X, Wang D, Xu F, Ding X, Zhang Z, Bi D, Cheng YT, Chen S, Li X, Zhang Y. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 2009 Jul 23;6(1):34-44. doi: 10.1016/j.chom.2009.05.019. PMID:19616764 doi:https://dx.doi.org/10.1016/j.chom.2009.05.019

[2] Leslie ME, Lewis MW, Youn JY, Daniels MJ, Liljegren SJ. The EVERSHED receptor-like kinase modulates floral organ shedding in Arabidopsis. Development. 2010 Feb;137(3):467-76. doi: 10.1242/dev.041335. PMID:20081191 doi:https://dx.doi.org/10.1242/dev.041335

[3] Albert I, Bohm H, Albert M, Feiler CE, Imkampe J, Wallmeroth N, Brancato C, Raaymakers TM, Oome S, Zhang H, Krol E, Grefen C, Gust AA, Chai J, Hedrich R, Van den Ackerveken G, Nurnberger T. An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nat Plants. 2015 Oct 5;1:15140. doi: 10.1038/nplants.2015.140. PMID:27251392 doi:https://dx.doi.org/10.1038/nplants.2015.140

[4] Hohmann U, Hothorn M. Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1. Acta Crystallogr D Struct Biol. 2019 May 1;75(Pt 5):488-497. doi:, 10.1107/S2059798319005291. Epub 2019 Apr 29. PMID:31063151 doi:https://dx.doi.org/10.1107/S2059798319005291

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[2]

[3]

[4]

@@ Line 3: / Line 3: @@
 <StructureSection load='6r1h' size='340' side='right'caption='[[6r1h]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6r1h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R1H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6r1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R1H FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SOBIR1, EVR, At2g31880, F20M17.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1h OCA], [http://pdbe.org/6r1h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r1h RCSB], [http://www.ebi.ac.uk/pdbsum/6r1h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1h ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1h OCA], [https://pdbe.org/6r1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r1h RCSB], [https://www.ebi.ac.uk/pdbsum/6r1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1h ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SBIR1_ARATH SBIR1_ARATH]] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death (PubMed:19616764). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity (PubMed:27251392). Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission (PubMed:20081191).<ref>PMID:19616764</ref> <ref>PMID:20081191</ref> <ref>PMID:27251392</ref>
+[https://www.uniprot.org/uniprot/SBIR1_ARATH SBIR1_ARATH] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death (PubMed:19616764). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity (PubMed:27251392). Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission (PubMed:20081191).<ref>PMID:19616764</ref> <ref>PMID:20081191</ref> <ref>PMID:27251392</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Hohmann, U]]
+[[Category: Hohmann U]]
-[[Category: Hothorn, M]]
+[[Category: Hothorn M]]
-[[Category: Immune system]]
-[[Category: Lrr receptor]]
-[[Category: Plant immunity]]
-[[Category: Receptor kinase]]
-[[Category: Receptor-like protein interacting kinase]]

6r1h: Difference between revisions

Latest revision as of 10:24, 23 October 2024

Crystal structure of the LRR ectodomain of the receptor kinase SOBIR1 from Arabidopsis thaliana.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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