|
|
| Line 3: |
Line 3: |
| <StructureSection load='7oc6' size='340' side='right'caption='[[7oc6]], [[Resolution|resolution]] 2.01Å' scene=''> | | <StructureSection load='7oc6' size='340' side='right'caption='[[7oc6]], [[Resolution|resolution]] 2.01Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7oc6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acremonium_strictum_(strain_imi_501407) Acremonium strictum (strain imi 501407)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OC6 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OC6 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asR6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2203296 Acremonium strictum (strain IMI 501407)])</td></tr>
| |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-humulene_synthase Alpha-humulene synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.104 4.2.3.104] </span></td></tr> | |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oc6 OCA], [https://pdbe.org/7oc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oc6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oc6 OCA], [https://pdbe.org/7oc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oc6 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
| |
| [[https://www.uniprot.org/uniprot/ASR6_SARSH ASR6_SARSH]] Alpha-humulene synthase; part of the gene cluster that mediates the biosynthesis of xenovulene A, an unusual meroterpenoid that has potent inhibitory effects on the human gamma-aminobutyrate A (GABAA) benzodiazepine receptor (PubMed:29773797). The first step of xenovulene A biosynthesis is the biosynthesis of 3-methylorcinaldehyde performed by the non-reducing polyketide synthase aspks1 (PubMed:17912413, PubMed:29773797, PubMed:20552126). The salicylate hydroxylase asL1 then catalyzes the oxidative dearomatization of 3-methylorcinaldehyde to yield a dearomatized hydroxycyclohexadione (PubMed:29773797). The 2-oxoglutarate-dependent dioxygenase asL3 further catalyzes the oxidative ring expansion to provide the first tropolone metabolite (PubMed:29773797). The cytochrome P450 monooxygenase asR2 allows the synthesis of tropolone hemiacetal (PubMed:29773797). In parallel, a previously unrecognised class of terpene cyclase, asR6, produces alpha-humulene from farnesylpyrophosphate (FPP) (PubMed:29773797). The putative Diels-Alderase asR5 probably catalyzes the formation of the tropolone-humulene skeleton by linking humulene and the polyketide moiety (PubMed:29773797). Oxidative-ring contractions catalyzed by asL4 and asL6 then processively remove carbon atoms from the polyketide to yield xenovulene A (PubMed:29773797).<ref>PMID:17912413</ref> <ref>PMID:20552126</ref> <ref>PMID:29773797</ref>
| |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 25: |
Line 21: |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Alpha-humulene synthase]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Blankenfeldt, W]] | | [[Category: Blankenfeldt W]] |
| [[Category: Cox, R J]] | | [[Category: Cox RJ]] |
| [[Category: Deuschmann, A]] | | [[Category: Deuschmann A]] |
| [[Category: Lukat, P]] | | [[Category: Lukat P]] |
| [[Category: Schotte, C]] | | [[Category: Schotte C]] |
| [[Category: 2e-humulene]]
| |
| [[Category: Alpha-humulene]]
| |
| [[Category: Biosynthetic protein]]
| |
| [[Category: Cyclase]]
| |
| [[Category: Humulene]]
| |
| [[Category: Terpene cyclase]]
| |
| [[Category: Tropolone sesquiterpenoid]]
| |
| [[Category: Xenovulene]]
| |
