8bzq

Beta-1,4-D-endoglucanase Cel45A from Gloeophyllum trabeum

spinqualitylabelsall models 8bzq, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bzq is a 1 chain structure with sequence from Gloeophyllum trabeum ATCC 11539. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function S7QB86_GLOTA Publication Abstract from PubMed Here we describe the first crystal structure of a beta-1,4-endoglucanase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A, which belongs to subfamily C of glycoside hydrolase family 45 (GH45). GtCel45A is ~ 18 kDa in size and the crystal structure contains 179 amino acids. The structure is refined at 1.30 A resolution and R(free) 0.18. The enzyme consists of a single catalytic module folded into a six-stranded double-psi beta-barrel domain surrounded by long loops. GtCel45A is very similar in sequence (82% identity) and structure to PcCel45A from the white-rot fungus Phanerochaete chrysosporium. Surprisingly though, initial hydrolysis of barley beta-glucan was almost twice as fast in GtCel45A as compared to PcCel45A. The first crystal structure of a family 45 glycoside hydrolase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A.,Okmane L, Fitkin L, Sandgren M, Stahlberg J FEBS Open Bio. 2024 Mar;14(3):505-514. doi: 10.1002/2211-5463.13774. Epub 2024 , Feb 4. PMID:38311343 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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