Net charge: Difference between revisions
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<br>You can calculate the net charge of your protein easily at a given pH or over a range: | <br>You can calculate the net charge of your protein easily at a given pH or over a range: | ||
== | ==Protein Calculator== | ||
#First, get the one-letter amino acid sequence of your protein of interest. | #First, get the one-letter amino acid sequence of your protein of interest. | ||
##See the [[Isoelectric point|Isoelectric point page]] for some methods. | ##See the [[Isoelectric point|Isoelectric point page]] for some methods. | ||
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#Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator]. | #Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator]. | ||
#Check the first box under Charge at the right, and click Submit Query. | #Check the first box under Charge at the right, and click Submit Query. | ||
==Prot-pi== | |||
"Prot pi | Protein Tool calculates isoelectric point and net charge of proteins, as well as the exact mass and the absorption coefficient using the amino acid sequence." | |||
*Go to [https://www.protpi.ch/Calculator/ProteinTool Prot-pi]] and follow instructions. | |||
==See Also== | ==See Also== | ||
