7v1n: Difference between revisions
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==== | ==Structure of the Clade 2 C. difficile TcdB in complex with its receptor TFPI== | ||
<StructureSection load='7v1n' size='340' side='right'caption='[[7v1n]]' scene=''> | <StructureSection load='7v1n' size='340' side='right'caption='[[7v1n]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7v1n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile Clostridioides difficile] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V1N FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v1n OCA], [https://pdbe.org/7v1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v1n RCSB], [https://www.ebi.ac.uk/pdbsum/7v1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v1n ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v1n OCA], [https://pdbe.org/7v1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v1n RCSB], [https://www.ebi.ac.uk/pdbsum/7v1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v1n ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/TCDB2_CLODI TCDB2_CLODI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The emergence of hypervirulent clade 2 Clostridioides difficile is associated with severe symptoms and accounts for >20% of global infections. TcdB is a dominant virulence factor of C. difficile, and clade 2 strains exclusively express two TcdB variants (TcdB2 and TcdB4) that use unknown receptors distinct from the classic TcdB. Here, we performed CRISPR/Cas9 screens for TcdB4 and identified tissue factor pathway inhibitor (TFPI) as its receptor. Using cryo-EM, we determined a complex structure of the full-length TcdB4 with TFPI, defining a common receptor-binding region for TcdB. Residue variations within this region divide major TcdB variants into 2 classes: one recognizes Frizzled (FZD), and the other recognizes TFPI. TFPI is highly expressed in the intestinal glands, and recombinant TFPI protects the colonic epithelium from TcdB2/4. These findings establish TFPI as a colonic crypt receptor for TcdB from clade 2 C. difficile and reveal new mechanisms for CDI pathogenesis. | |||
TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile.,Luo J, Yang Q, Zhang X, Zhang Y, Wan L, Zhan X, Zhou Y, He L, Li D, Jin D, Zhen Y, Huang J, Li Y, Tao L Cell. 2022 Mar 17;185(6):980-994.e15. doi: 10.1016/j.cell.2022.02.010. PMID:35303428<ref>PMID:35303428</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7v1n" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Tissue factor pathway inhibitor|Tissue factor pathway inhibitor]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Clostridioides difficile]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Li Y]] | ||
[[Category: Luo J]] | |||
[[Category: Tao L]] | |||
[[Category: Wan L]] | |||
[[Category: Yang Q]] | |||
[[Category: Zhang X]] | |||
[[Category: Zhang Y]] | |||
Latest revision as of 11:44, 30 October 2024
Structure of the Clade 2 C. difficile TcdB in complex with its receptor TFPI
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