7vy2: Difference between revisions
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==== | ==STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF RHODOBACTER SPHAEROIDES DIMER== | ||
<StructureSection load='7vy2' size='340' side='right'caption='[[7vy2]]' scene=''> | <StructureSection load='7vy2' size='340' side='right'caption='[[7vy2]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7vy2]] is a 66 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_f._sp._denitrificans Cereibacter sphaeroides f. sp. denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VY2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vy2 OCA], [https://pdbe.org/7vy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vy2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vy2 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PGV:(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(11E)-OCTADEC-11-ENOATE'>PGV</scene>, <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vy2 OCA], [https://pdbe.org/7vy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vy2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vy2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Rhodobacter sphaeroides is a model organism in bacterial photosynthesis, and its light-harvesting-reaction center (LH1-RC) complex contains both dimeric and monomeric forms. Here we present cryo-EM structures of the native LH1-RC dimer and an LH1-RC monomer lacking protein-U (DeltaU). The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. PufX plays a critical role in connecting the two monomers in a dimer, with one PufX interacting at its N-terminus with another PufX and an LH1 beta-polypeptide in the other monomer. One protein-U was only partially resolved in the dimeric structure, signaling different degrees of disorder in the two monomers. The DeltaU LH1-RC monomer was half-moon-shaped and contained 11 alpha- and 10 beta-polypeptides, indicating a critical role for protein-U in controlling the number of alphabeta-subunits required for dimer assembly and stabilization. These features are discussed in relation to membrane topology and an assembly model proposed for the native dimeric complex. | |||
Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex.,Tani K, Kanno R, Kikuchi R, Kawamura S, Nagashima KVP, Hall M, Takahashi A, Yu LJ, Kimura Y, Madigan MT, Mizoguchi A, Humbel BM, Wang-Otomo ZY Nat Commun. 2022 Apr 7;13(1):1904. doi: 10.1038/s41467-022-29453-8. PMID:35393413<ref>PMID:35393413</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7vy2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Cereibacter sphaeroides f. sp. denitrificans]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Z- | [[Category: Hall M]] | ||
[[Category: Humbel BM]] | |||
[[Category: Kanno R]] | |||
[[Category: Kawamura S]] | |||
[[Category: Kikuchi R]] | |||
[[Category: Kimura Y]] | |||
[[Category: Madigan MT]] | |||
[[Category: Mizoguchi A]] | |||
[[Category: Nagashima KVP]] | |||
[[Category: Takahashi A]] | |||
[[Category: Tani K]] | |||
[[Category: Wang-Otomo Z-Y]] | |||
[[Category: Yu L-J]] | |||
Latest revision as of 11:45, 30 October 2024
STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF RHODOBACTER SPHAEROIDES DIMER
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