8rot: Difference between revisions
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==Cryo-EM structure of the transmembrane anti-sigma factor DdvA== | |||
<StructureSection load='8rot' size='340' side='right'caption='[[8rot]], [[Resolution|resolution]] 3.11Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8rot]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ROT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ROT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.11Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rot OCA], [https://pdbe.org/8rot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rot RCSB], [https://www.ebi.ac.uk/pdbsum/8rot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rot ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q1CW22_MYXXD Q1CW22_MYXXD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS and one type III-B CRISPR-Cas system, regulated by the transmembrane anti-sigma DdvA and its cognate extracytoplasmic function (ECF) sigma DdvS, can defend Myxococcus xanthus against a phage. Cryo-electron microscopy reveals DdvA-DdvS pairs assemble as arrow-shaped transmembrane dimers. Each DdvA periplasmic domain adopts a separase/craspase-type tetratricopeptide repeat (TPR)-caspase HetF-associated with TPR (TPR-CHAT) architecture with an incomplete His-Cys active site, lacking three alpha-helices conserved among CHAT domains. Each active site faces the dimer interface, raising the possibility that signal-induced caspase-like DdvA autoproteolysis in trans precedes RseP-mediated intramembrane proteolysis and DdvS release. Nuclear magnetic resonance reveals a DdvA cytoplasmic CHCC-type zinc-bound three-helix bundle that binds to DdvS sigma(2) and sigma(4) domains, undergoing sigma(4)-induced helix extension to trap DdvS. Altogether, we provide structural-mechanistic insights into membrane anti-sigma-ECF sigma regulation of an antiviral CBASS-CRISPR-Cas defense island. | |||
Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-sigma factor and its ECF sigma partner.,Bernal-Bernal D, Pantoja-Uceda D, Lopez-Alonso JP, Lopez-Rojo A, Lopez-Ruiz JA, Galbis-Martinez M, Ochoa-Lizarralde B, Tascon I, Elias-Arnanz M, Ubarretxena-Belandia I, Padmanabhan S Sci Adv. 2024 Oct 25;10(43):eadp1053. doi: 10.1126/sciadv.adp1053. Epub 2024 Oct , 25. PMID:39454004<ref>PMID:39454004</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8rot" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Myxococcus xanthus]] | |||
[[Category: Lopez-Alonso JP]] | |||
[[Category: Ochoa-Lizarralde B]] | |||
[[Category: Tascon I]] | |||
[[Category: Ubarretxena-Belandia I]] | |||
Latest revision as of 08:06, 6 November 2024
Cryo-EM structure of the transmembrane anti-sigma factor DdvA
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