2v0p: Difference between revisions

Latest revision as of 09:31, 6 November 2024

The Structure of Tap42 Alpha4 Subunit

Structural highlights

2v0p is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	MSE, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TAP42_YEAST Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Physiological functions of protein phosphatase 2A (PP2A) are determined via the association of its catalytic subunit (PP2Ac) with diverse regulatory subunits. The predominant form of PP2Ac assembles into a heterotrimer comprising the scaffolding PR65/A subunit together with a variable regulatory B subunit. A distinct population of PP2Ac associates with the Tap42/alpha4 subunit, an interaction mutually exclusive with that of PR65/A. Tap42/alpha4 is also an interacting subunit of the PP2Ac-related phosphatases, PP4 and PP6. Tap42/alpha4, an essential protein in yeast and suppressor of apoptosis in mammals, contributes to critical cellular functions including the Tor signaling pathway. Here, we describe the crystal structure of the PP2Ac-interaction domain of Saccharomyces cerevisiae Tap42. The structure reveals an all alpha-helical protein with striking similarity to 14-3-3 and tetratricopeptide repeat (TPR) proteins. Mutational analyses of structurally conserved regions of Tap42/alpha4 identified a positively charged region critical for its interactions with PP2Ac. We propose a scaffolding function for Tap42/alpha4 whereby the interaction of PP2Ac at its N-terminus promotes the dephosphorylation of substrates recruited to the C-terminal region of the molecule.

The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation.,Yang J, Roe SM, Prickett TD, Brautigan DL, Barford D Biochemistry. 2007 Jul 31;46(30):8807-15. Epub 2007 Jul 6. PMID:17616149^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang H, Wang X, Jiang Y. Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases. Mol Biol Cell. 2003 Nov;14(11):4342-51. Epub 2003 Jul 25. PMID:14551259 doi:https://dx.doi.org/10.1091/mbc.E03-02-0072
↑ Duvel K, Santhanam A, Garrett S, Schneper L, Broach JR. Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast. Mol Cell. 2003 Jun;11(6):1467-78. PMID:12820961
↑ Wang H, Jiang Y. The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast. Mol Cell Biol. 2003 May;23(9):3116-25. PMID:12697813
↑ Santhanam A, Hartley A, Duvel K, Broach JR, Garrett S. PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p. Eukaryot Cell. 2004 Oct;3(5):1261-71. PMID:15470255 doi:https://dx.doi.org/10.1128/EC.3.5.1261-1271.2004
↑ Yang J, Roe SM, Prickett TD, Brautigan DL, Barford D. The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation. Biochemistry. 2007 Jul 31;46(30):8807-15. Epub 2007 Jul 6. PMID:17616149 doi:10.1021/bi7007118

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Wang H, Wang X, Jiang Y. Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases. Mol Biol Cell. 2003 Nov;14(11):4342-51. Epub 2003 Jul 25. PMID:14551259 doi:https://dx.doi.org/10.1091/mbc.E03-02-0072

[2] Duvel K, Santhanam A, Garrett S, Schneper L, Broach JR. Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast. Mol Cell. 2003 Jun;11(6):1467-78. PMID:12820961

[3] Wang H, Jiang Y. The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast. Mol Cell Biol. 2003 May;23(9):3116-25. PMID:12697813

[4] Santhanam A, Hartley A, Duvel K, Broach JR, Garrett S. PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p. Eukaryot Cell. 2004 Oct;3(5):1261-71. PMID:15470255 doi:https://dx.doi.org/10.1128/EC.3.5.1261-1271.2004

[5] Yang J, Roe SM, Prickett TD, Brautigan DL, Barford D. The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation. Biochemistry. 2007 Jul 31;46(30):8807-15. Epub 2007 Jul 6. PMID:17616149 doi:10.1021/bi7007118

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@@ Line 3: / Line 3: @@
 <StructureSection load='2v0p' size='340' side='right'caption='[[2v0p]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V0P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V0P FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v0p OCA], [https://pdbe.org/2v0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v0p RCSB], [https://www.ebi.ac.uk/pdbsum/2v0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v0p ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/TAP42_YEAST TAP42_YEAST]] Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.<ref>PMID:14551259</ref> <ref>PMID:12820961</ref> <ref>PMID:12697813</ref> <ref>PMID:15470255</ref>
+[https://www.uniprot.org/uniprot/TAP42_YEAST TAP42_YEAST] Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.<ref>PMID:14551259</ref> <ref>PMID:12820961</ref> <ref>PMID:12697813</ref> <ref>PMID:15470255</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
    <jmolCheckbox>
      <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v0/2v0p_consurf.spt"</scriptWhenChecked>
-     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Barford, D]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Roe, S M]]
+[[Category: Barford D]]
-[[Category: Yang, J]]
+[[Category: Roe SM]]
-[[Category: Hydrolase inhibitor]]
+[[Category: Yang J]]
-[[Category: Phosphorylation]]
-[[Category: Signal transduction inhibitor]]

2v0p: Difference between revisions

Latest revision as of 09:31, 6 November 2024

The Structure of Tap42 Alpha4 Subunit

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

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