7waa: Difference between revisions
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==Crystal structure of MCR-1-S treated by AgNO3== | ==Crystal structure of MCR-1-S treated by AgNO3== | ||
<StructureSection load='7waa' size='340' side='right'caption='[[7waa]]' scene=''> | <StructureSection load='7waa' size='340' side='right'caption='[[7waa]], [[Resolution|resolution]] 1.58Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAA FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAA FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7waa OCA], [https://pdbe.org/7waa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7waa RCSB], [https://www.ebi.ac.uk/pdbsum/7waa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7waa ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7waa OCA], [https://pdbe.org/7waa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7waa RCSB], [https://www.ebi.ac.uk/pdbsum/7waa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7waa ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Significance Superbugs carrying a mobile colistin resistance gene (mcr) are jeopardizing the clinical efficacy of the last-line antibiotic colistin. The development of MCR inhibitors is urgently required to cope with antibiotic-resistance emergencies. Here, we show that silver (Ag(+)) fully restores the susceptibility of mcr-1-carrying superbugs against colistin both in vitro and in vivo. We found an unprecedented tetra-silver center in the active-site pocket of MCR-1 through the substitution of the essential Zn(2+) ions in the intact enzyme, leading to the prevention of substrate binding (i.e. the dysfunction of MCR-1 in transferring phosphorylethanolamine to lipid A). Importantly, the ability of Ag(+) to suppress resistance evolution extends the lifespan of currently used antibiotics, providing a strategy to treat infections by mcr-positive bacteria. | |||
Re-sensitization of mcr carrying multidrug resistant bacteria to colistin by silver.,Zhang Q, Wang R, Wang M, Liu C, Koohi-Moghadam M, Wang H, Ho PL, Li H, Sun H Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2119417119. doi:, 10.1073/pnas.2119417119. Epub 2022 Mar 9. PMID:35263219<ref>PMID:35263219</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7waa" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 14:09, 6 November 2024
Crystal structure of MCR-1-S treated by AgNO3
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