8txb

Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.9A

spinqualitylabelsall models 8txb, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8txb is a 4 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A8J9H7_CHLRE Publication Abstract from PubMed Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the channel protein PKD2, implying their potential contribution to external signal sensing and flagellar motility control. Here, we present the single-particle cryo-electron microscopy structure of the mastigoneme at 3.4 A. The filament unit, MST1, consists of nine immunoglobulin-like domains and six Sushi domains, trailed by an elastic polyproline-II helix. Our structure demonstrates that MST1 subunits are periodically assembled to form a centrosymmetric, non-polar filament. Intriguingly, numerous clustered disulfide bonds within a ladder-like spiral configuration underscore structural resilience. While defects in the mastigoneme structure did not noticeably affect general attributes of cell swimming, they did impact specific swimming properties, particularly under varied environmental conditions such as redox shifts and heightened viscosity. Our findings illuminate the potential role of mastigonemes in flagellar motility and suggest their involvement in diverse environmental responses. Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.,Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K J Cell Biol. 2023 Dec 4;222(12):e202301066. doi: 10.1083/jcb.202301066. Epub 2023 , Oct 26. PMID:37882754 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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