How to predict structures with AlphaFold: Difference between revisions
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==Instructions for ColabFold== | ==Instructions for ColabFold== | ||
This procedure was written in 2022<ref name="">Some of the recommended options were gleaned from the [https://www.youtube.com/watch?v=Rfw7thgGTwI 1 hour 46 minute video of presentations by Sergey Ovchinnikov and Martin Steinegger] (August, 2021) for the Boston Protein Design and Modeling Club | |||
hosted by Chris Bahl. | hosted by Chris Bahl. | ||
</ref>. In 2024, ColabFold is not necessarily the best or only place to submit you job: see [[#Prediction Servers]]. | |||
Initially, AlphaFold and ColabFold performed best with '''single chains'''<ref name="complexes">[https://www.biorxiv.org/content/10.1101/2021.10.04.463034v1 Protein complex prediction with AlphaFold-Multimer], 2021, Evans ''et al.'' (DeepMind Team).</ref>, which may include one or a few domains. The instructions below were written '''before ColabFold was adapted to prediction of multimers'''. If you are interested in complexes or alternate conformations, please see ColabFold instructions in the 2023 paper by Kim ''et al.'' <ref name="kim2023">[https://protocolexchange.researchsquare.com/article/pex-2490/v1 Easy and accurate protein structure prediction using ColabFold], 2023, Kim ''et al.'' (DeepMind Team).</ref> | Initially, AlphaFold and ColabFold performed best with '''single chains'''<ref name="complexes">[https://www.biorxiv.org/content/10.1101/2021.10.04.463034v1 Protein complex prediction with AlphaFold-Multimer], 2021, Evans ''et al.'' (DeepMind Team).</ref>, which may include one or a few domains. The instructions below were written '''before ColabFold was adapted to prediction of multimers'''. If you are interested in complexes or alternate conformations, please see ColabFold instructions in the 2023 paper by Kim ''et al.'' <ref name="kim2023">[https://protocolexchange.researchsquare.com/article/pex-2490/v1 Easy and accurate protein structure prediction using ColabFold], 2023, Kim ''et al.'' (DeepMind Team).</ref> | ||
