6bt9

Chitinase ChiA74 from Bacillus thuringiensis

spinqualitylabelsall models 6bt9, resolution 2.26Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6bt9 is a 2 chain structure with sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.26Å Ligands: CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed There is no structural information about any chitinase synthesized by Bacillus thuringiensis, the most successful microbial insect larvicide used worldwide. In this study, we solved the 3D structure of the chitinase ChiA74 at 2.26 A. The crystal structure shows that ChiA74 is composed of a modular arrangement formed by (i) a catalytic region (CD), (ii) a chitinase insertion domain (CID), (iii) a fibronectin type III domain (FnIII), and (iv) a chitin binding domain (CBD). The location of the CBD with respect to the CD has no structural similarity to other chitinases with known structures. The activity of a ChiA74 lacking its secretion signal peptide (ChiA74Deltasp) and a truncated version lacking its CBD/FnIII domains (ChiA74Deltasp-50) did not have statistical differences in activity against colloidal chitin. However, ChiA74Deltasp exhibits 4.5 and 2.0 higher activity than versions lacking the CBD (ChiA74Deltasp-60) and CBD/FnIII domains (ChiA74Deltasp-50), respectively, when crystalline chitin was used as substrate. Our data suggest that the CBD might plays a significant role in crystalline chitin hydrolysis. We also demonstrated the importance of the catalytic E211 in the CD, as mutants ChiA74DeltaspE211N and ChiA74DeltaspD207N, E211N were inactive against colloidal and crystalline chitins, chitosan and 4-MU-GlcNAc3. ChiA74 has a processive activity producing oligosaccharides with degree of polymerization (DP) of 1 (GlcNAc) and 2 (GlcNAc2). The crystal structure of the chitinase ChiA74 of Bacillus thuringiensis has a multidomain assembly.,Juarez-Hernandez EO, Casados-Vazquez LE, Brieba LG, Torres-Larios A, Jimenez-Sandoval P, Barboza-Corona JE Sci Rep. 2019 Feb 22;9(1):2591. doi: 10.1038/s41598-019-39464-z. PMID:30796308 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Chitinase 3D structures

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