6hug: Difference between revisions

Latest revision as of 05:19, 21 November 2024

CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38.

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Structural highlights

6hug is a 6 chain structure with sequence from Bos taurus, Homo sapiens and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.1Å
Ligands:	BMA, MAN, NAG, PIO, RI5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GBRA1_BOVIN Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.^[1]

Publication Abstract from PubMed

Type-A gamma-aminobutyric (GABAA) receptors are ligand-gated chloride channels with a very rich pharmacology. Some of their modulators, including benzodiazepines and general anaesthetics, are among the most successful drugs in clinical use and are common substances of abuse. Without reliable structural data, the mechanistic basis for the pharmacological modulation of GABAA receptors remains largely unknown. Here we report several high-resolution cryo-electron microscopy structures in which the full-length human alpha1beta3gamma2L GABAA receptor in lipid nanodiscs is bound to the channel-blocker picrotoxin, the competitive antagonist bicuculline, the agonist GABA (gamma-aminobutyric acid), and the classical benzodiazepines alprazolam and diazepam. We describe the binding modes and mechanistic effects of these ligands, the closed and desensitized states of the GABAA receptor gating cycle, and the basis for allosteric coupling between the extracellular, agonist-binding region and the transmembrane, pore-forming region. This work provides a structural framework in which to integrate previous physiology and pharmacology research and a rational basis for the development of GABAA receptor modulators.

GABAA receptor signalling mechanisms revealed by structural pharmacology.,Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR Nature. 2019 Jan 2. pii: 10.1038/s41586-018-0832-5. doi:, 10.1038/s41586-018-0832-5. PMID:30602790^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schofield PR, Darlison MG, Fujita N, Burt DR, Stephenson FA, Rodriguez H, Rhee LM, Ramachandran J, Reale V, Glencorse TA, et al.. Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family. Nature. 1987 Jul 16-22;328(6127):221-7. PMID:3037384 doi:https://dx.doi.org/10.1038/328221a0
↑ Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR. GABAA receptor signalling mechanisms revealed by structural pharmacology. Nature. 2019 Jan 2. pii: 10.1038/s41586-018-0832-5. doi:, 10.1038/s41586-018-0832-5. PMID:30602790 doi:https://dx.doi.org/10.1038/s41586-018-0832-5

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Schofield PR, Darlison MG, Fujita N, Burt DR, Stephenson FA, Rodriguez H, Rhee LM, Ramachandran J, Reale V, Glencorse TA, et al.. Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family. Nature. 1987 Jul 16-22;328(6127):221-7. PMID:3037384 doi:https://dx.doi.org/10.1038/328221a0

[2] Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR. GABAA receptor signalling mechanisms revealed by structural pharmacology. Nature. 2019 Jan 2. pii: 10.1038/s41586-018-0832-5. doi:, 10.1038/s41586-018-0832-5. PMID:30602790 doi:https://dx.doi.org/10.1038/s41586-018-0832-5

[1]

[2]

@@ Line 3: / Line 3: @@
 <SX load='6hug' size='340' side='right' viewer='molstar' caption='[[6hug]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6hug]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin], [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HUG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6hug]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HUG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene>, <scene name='pdbligand=RI5:(1AR,2AR,3S,6R,6AS,8AS,8BR,9R)-2A-HYDROXY-8B-METHYL-9-(PROP-1-EN-2-YL)HEXAHYDRO-3,6-METHANO-1,5,7-TRIOXACYCLOPENTA[IJ]CYCLOPROPA[A]AZULENE-4,8(3H)-DIONE'>RI5</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GABRA1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), GABRB3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), GABRG2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene>, <scene name='pdbligand=RI5:(1AR,2AR,3S,6R,6AS,8AS,8BR,9R)-2A-HYDROXY-8B-METHYL-9-(PROP-1-EN-2-YL)HEXAHYDRO-3,6-METHANO-1,5,7-TRIOXACYCLOPENTA[IJ]CYCLOPROPA[A]AZULENE-4,8(3H)-DIONE'>RI5</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hug OCA], [https://pdbe.org/6hug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hug RCSB], [https://www.ebi.ac.uk/pdbsum/6hug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hug ProSAT]</span></td></tr>
 </table>
-== Disease ==
-[[https://www.uniprot.org/uniprot/GBRG2_HUMAN GBRG2_HUMAN]] Childhood absence epilepsy;Dravet syndrome;Generalized epilepsy with febrile seizures-plus. Disease susceptibility is associated with variations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry. [[https://www.uniprot.org/uniprot/GBRB3_HUMAN GBRB3_HUMAN]] Autism;Childhood absence epilepsy. Disease susceptibility is associated with variations affecting the gene represented in this entry.
 == Function ==
-[[https://www.uniprot.org/uniprot/GBRA1_BOVIN GBRA1_BOVIN]] Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.<ref>PMID:3037384</ref>  [[https://www.uniprot.org/uniprot/GBRG2_HUMAN GBRG2_HUMAN]] Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.<ref>PMID:2538761</ref>  [[https://www.uniprot.org/uniprot/GBRB3_HUMAN GBRB3_HUMAN]] GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
+[https://www.uniprot.org/uniprot/GBRA1_BOVIN GBRA1_BOVIN] Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.<ref>PMID:3037384</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 26: @@
 __TOC__
 </SX>
-[[Category: Bovin]]
+[[Category: Bos taurus]]
-[[Category: Camelus glama]]
+[[Category: Homo sapiens]]
-[[Category: Human]]
+[[Category: Lama glama]]
 [[Category: Large Structures]]
-[[Category: Aricescu, A R]]
+[[Category: Aricescu AR]]
-[[Category: Desai, R]]
+[[Category: Desai R]]
-[[Category: Jasenko, Z]]
+[[Category: Jasenko Z]]
-[[Category: Karia, D]]
+[[Category: Karia D]]
-[[Category: Kotecha, A]]
+[[Category: Kotecha A]]
-[[Category: Laverty, D]]
+[[Category: Laverty D]]
-[[Category: Malinauskas, T]]
+[[Category: Malinauskas T]]
-[[Category: Martin, I Serna]]
+[[Category: Masiulis S]]
-[[Category: Masiulis, S]]
+[[Category: Miller KW]]
-[[Category: Miller, K W]]
+[[Category: Pardon E]]
-[[Category: Pardon, E]]
+[[Category: Serna Martin I]]
-[[Category: Steyaert, J]]
+[[Category: Steyaert J]]
-[[Category: Uchanski, T]]
+[[Category: Uchanski T]]
-[[Category: Channel]]
-[[Category: Chloride]]
-[[Category: Cn]]
-[[Category: Cys-loop]]
-[[Category: Gaba]]
-[[Category: Gabaar]]
-[[Category: Inhibition]]
-[[Category: Ion]]
-[[Category: Megabody]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Nanobody]]
-[[Category: Neuron]]
-[[Category: Picrotoxin]]
-[[Category: Plgic]]
-[[Category: Ptx]]
-[[Category: Signalling]]

6hug: Difference between revisions

Latest revision as of 05:19, 21 November 2024

CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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