6iwk

The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07

spinqualitylabelsall models 6iwk, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6iwk is a 1 chain structure with sequence from Pelomonas saccharophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.501Å Ligands: CA, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function AMT4_ROSSA Publication Abstract from PubMed The maltooligosaccharide-forming amylases (MFAses) degrade starch into maltooligosaccharides which potentially benefit human diet and grow popular in food processing, but little has been studied about their product specificity and structures. We focused on this topic and provide evidence through an X-ray crystal structure of the maltotetraose (G4)-forming amylase from Pseudomonas saccharophila STB07 (MFAps), as well as co-crystal structures of MFAps with G4 and with pseudo-maltoheptaose (pseudo-G7) determined at up to 1.1 A resolution. G4 and pseudo-G7 occupy active cleft subsites -4 to -1 and -4 to +3 respectively. Binding induces conformational changes in the active sites except Asp193, working as the base catalyst. Comparison of the MFAps structure with those of other alpha-amylases revealed obvious differences in the loop structures providing dominant interactions between protein and substrate in the non-reducing side of the active sites cleft. These structures at the non-reducing end may govern the G4 specificity of MFAps and also be relevant to its exo-type action pattern. Structure of maltotetraose-forming amylase from Pseudomonas saccharophila STB07 provides insights into its product specificity.,Zhang Z, Jin T, Xie X, Ban X, Li C, Hong Y, Cheng L, Gu Z, Li Z Int J Biol Macromol. 2019 Nov 18. pii: S0141-8130(19)37071-0. doi:, 10.1016/j.ijbiomac.2019.11.006. PMID:31751711 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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