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<StructureSection load='6wby' size='340' side='right'caption='[[6wby]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
<StructureSection load='6wby' size='340' side='right'caption='[[6wby]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6wby]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_abcessus"_moore_and_frerichs_1953 "mycobacterium abcessus" moore and frerichs 1953]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WBY FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WBY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6w98|6w98]], [[6wbx|6wbx]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D2E76_26050 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36809 "Mycobacterium abcessus" Moore and Frerichs 1953])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wby OCA], [https://pdbe.org/6wby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wby RCSB], [https://www.ebi.ac.uk/pdbsum/6wby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wby ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wby OCA], [https://pdbe.org/6wby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wby RCSB], [https://www.ebi.ac.uk/pdbsum/6wby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wby ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium tuberculosis causes tuberculosis, a disease that kills over 1 million people each year. Its cell envelope is a common antibiotic target and has a unique structure due, in part, to two lipidated polysaccharides-arabinogalactan and lipoarabinomannan. Arabinofuranosyltransferase D (AftD) is an essential enzyme involved in assembling these glycolipids. We present the 2.9-A resolution structure of M. abscessus AftD, determined by single-particle cryo-electron microscopy. AftD has a conserved GT-C glycosyltransferase fold and three carbohydrate-binding modules. Glycan array analysis shows that AftD binds complex arabinose glycans. Additionally, AftD is non-covalently complexed with an acyl carrier protein (ACP). 3.4- and 3.5-A structures of a mutant with impaired ACP binding reveal a conformational change, suggesting that ACP may regulate AftD function. Mutagenesis experiments using a conditional knockout constructed in M. smegmatis confirm the essentiality of the putative active site and the ACP binding for AftD function.
Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria.,Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F Mol Cell. 2020 May 5. pii: S1097-2765(20)30256-2. doi:, 10.1016/j.molcel.2020.04.014. PMID:32386575<ref>PMID:32386575</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6wby" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Mycobacterium abcessus moore and frerichs 1953]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Archer, M]]
[[Category: Archer M]]
[[Category: Athayde, D]]
[[Category: Athayde D]]
[[Category: Brunton, R]]
[[Category: Brunton R]]
[[Category: Carragher, B]]
[[Category: Carragher B]]
[[Category: Catalao, M J]]
[[Category: Catalao MJ]]
[[Category: Clarke, O B]]
[[Category: Clarke OB]]
[[Category: Dandey, V P]]
[[Category: Dandey VP]]
[[Category: Giacometti, S I]]
[[Category: Giacometti SI]]
[[Category: Kloss, B]]
[[Category: Kloss B]]
[[Category: Lowary, T L]]
[[Category: Lowary TL]]
[[Category: Mancia, F]]
[[Category: Mancia F]]
[[Category: Niederweis, M]]
[[Category: Niederweis M]]
[[Category: Pimentel, M]]
[[Category: Pimentel M]]
[[Category: Potter, C S]]
[[Category: Potter CS]]
[[Category: Raczkowski, A M]]
[[Category: Raczkowski AM]]
[[Category: Rodrigues, J]]
[[Category: Rodrigues J]]
[[Category: Rosario, A L]]
[[Category: Rosario AL]]
[[Category: Tan, Y Z]]
[[Category: Tan YZ]]
[[Category: Wei, H]]
[[Category: Wei H]]
[[Category: Zhang, L]]
[[Category: Zhang L]]
[[Category: Zheng, R B]]
[[Category: Zheng RB]]
[[Category: Acyl carrier protein]]
[[Category: Glycosyltransferase]]
[[Category: Membrane protein]]
[[Category: Nanodisc]]
Retrieved from "https://proteopedia.org/6wby"