8qma: Difference between revisions

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</table>

== Function ==

[https://www.uniprot.org/uniprot/~~A0A6C0M5W1_SINAL A0A6C0M5W1_SINAL~~] ~~DNA~~-~~dependent~~ RNA polymerase ~~catalyzes~~ the transcription of ~~DNA~~ into RNA ~~using the four ribonucleoside triphosphates as substrates~~.~~[ARBA~~:~~ARBA00004026][HAMAP~~-~~Rule~~:~~MF_01321][RuleBase~~:~~RU363031]~~

[https://www.uniprot.org/uniprot/RPOB_SINAL RPOB_SINAL]

== Publication Abstract from PubMed ==

Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.

Structure of the multi-subunit chloroplast RNA polymerase.,do Prado PFV, Ahrens FM, Liebers M, Ditz N, Braun HP, Pfannschmidt T, Hillen HS Mol Cell. 2024 Mar 7;84(5):910-925.e5. doi: 10.1016/j.molcel.2024.02.003. Epub , 2024 Feb 29. PMID:38428434<ref>PMID:38428434</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

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 </table>
 == Function ==
-[https://www.uniprot.org/uniprot/A0A6C0M5W1_SINAL A0A6C0M5W1_SINAL] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[ARBA:ARBA00004026][HAMAP-Rule:MF_01321][RuleBase:RU363031]
+[https://www.uniprot.org/uniprot/RPOB_SINAL RPOB_SINAL]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.
+Structure of the multi-subunit chloroplast RNA polymerase.,do Prado PFV, Ahrens FM, Liebers M, Ditz N, Braun HP, Pfannschmidt T, Hillen HS Mol Cell. 2024 Mar 7;84(5):910-925.e5. doi: 10.1016/j.molcel.2024.02.003. Epub , 2024 Feb 29. PMID:38428434<ref>PMID:38428434</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 8qma" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>