8uel

Crystal structure of enolase from Litopenaeus vannamei

spinqualitylabelsall models 8uel, resolution 2.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8uel is a 2 chain structure with sequence from Penaeus vannamei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.49Å Ligands: MG, PEP, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O96656_PENMO Publication Abstract from PubMed Transcriptome analysis had recognized enolase from shrimp Litopenaeus vannamei (L. vannamei), which is termed LvEnolase, as one of the allergens, but its amino acid sequence and protein structure have been lacking. In this study, natural LvEnolase was isolated from L. vannamei and characterized for the first time. The full-length cDNA sequence of LvEnolase was effectively cloned, which encoded 434 amino acid residues. The crystal structure of LvEnolase was successfully determined at a resolution of 2.5 A by X-ray crystallography (PDB: 8UEL). Notably, it was observed that near the active center, a loop exists in either an open or closed state, and the open loop was associated with the product release phase. Furthermore, enzyme activity assays were conducted to validate the catalytic capabilities of purified LvEnolase. These findings significantly enhance our comprehension of the enolase family and provide valuable support for delving into the functions and characteristics of LvEnolase. Characterization and Structural Analyses of Enolase from Shrimp (Litopenaeus vannamei).,Chang X, Zhang T, Zang J, Lv C, Zhao G J Agric Food Chem. 2023 Dec 13;71(49):19783-19790. doi: 10.1021/acs.jafc.3c07135. , Epub 2023 Nov 30. PMID:38033172 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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