3jz3: Difference between revisions

Latest revision as of 06:22, 27 November 2024

Structure of the cytoplasmic segment of histidine kinase QseC

Structural highlights

3jz3 is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	MSE, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

QSEC_ECOLI Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

QseC is a histidine kinase (HK) receptor involved in quorum sensing, a mechanism by which bacteria respond to fluctuations in cell population. We conducted a structural study of the cytoplasmic domain of QseC (QseC-CD) using X-ray crystallography. The 2.5 A structure of the apo-enzyme revealed that the kinase domain of QseC retains the overall fold of the typical HK kinase domain. The construct that we used is inactive in the autokinase reaction and its inactivity is most likely caused by its atypical dimerization interface, as compared to that observed in the T.maritima HK cytoplasmic domain structure. Restoration of the activity may require that the entire dimerization domain be present in the protein construct. QseC, which plays an important role in bacterial pathogenesis, is a promising drug target and the structure of QseC-CD provides a platform for developing more potent inhibitors of pathogen virulence.

Structure of the Cytoplasmic Segment of Histidine Kinase Receptor QseC, a Key Player in Bacterial Virulence.,Xie W, Dickson C, Kwiatkowski W, Choe S Protein Pept Lett. 2010 Jul 1. PMID:20594156^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sperandio V, Torres AG, Kaper JB. Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol. 2002 Feb;43(3):809-21. PMID:11929534
↑ Xie W, Dickson C, Kwiatkowski W, Choe S. Structure of the Cytoplasmic Segment of Histidine Kinase Receptor QseC, a Key Player in Bacterial Virulence. Protein Pept Lett. 2010 Jul 1. PMID:20594156

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Sperandio V, Torres AG, Kaper JB. Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol. 2002 Feb;43(3):809-21. PMID:11929534

[2] Xie W, Dickson C, Kwiatkowski W, Choe S. Structure of the Cytoplasmic Segment of Histidine Kinase Receptor QseC, a Key Player in Bacterial Virulence. Protein Pept Lett. 2010 Jul 1. PMID:20594156

[1]

[2]

@@ Line 1: / Line 1: @@
 ==Structure of the cytoplasmic segment of histidine kinase QseC==
-<StructureSection load='3jz3' size='340' side='right' caption='[[3jz3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='3jz3' size='340' side='right'caption='[[3jz3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3jz3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JZ3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3jz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JZ3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">qseC, ygiY, b3026, JW2994 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jz3 OCA], [https://pdbe.org/3jz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jz3 RCSB], [https://www.ebi.ac.uk/pdbsum/3jz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jz3 ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jz3 OCA], [http://pdbe.org/3jz3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jz3 RCSB], [http://www.ebi.ac.uk/pdbsum/3jz3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jz3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI]] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>
+[https://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 17: / Line 15: @@
    <jmolCheckbox>
      <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/3jz3_consurf.spt"</scriptWhenChecked>
-     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
@@ Line 35: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Histidine kinase]]
+[[Category: Large Structures]]
-[[Category: CSMP, Center for Structures of Membrane Proteins]]
+[[Category: Choe S]]
-[[Category: Choe, S]]
+[[Category: Kwiatkowski W]]
-[[Category: Kwiatkowski, W]]
+[[Category: Xie W]]
-[[Category: Xie, W]]
-[[Category: Atp-binding]]
-[[Category: Cell inner membrane]]
-[[Category: Cell membrane]]
-[[Category: Center for structures of membrane protein]]
-[[Category: Csmp]]
-[[Category: Helix-turn-helix]]
-[[Category: Kinase]]
-[[Category: Kinase domain]]
-[[Category: Membrane]]
-[[Category: Nucleotide-binding]]
-[[Category: Phosphoprotein]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Structural genomic]]
-[[Category: Transferase]]
-[[Category: Transmembrane]]
-[[Category: Two-component regulatory system]]

3jz3: Difference between revisions

Latest revision as of 06:22, 27 November 2024

Structure of the cytoplasmic segment of histidine kinase QseC

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

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