1umg: Difference between revisions
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'''Crystal strucure of fructose-1,6-bisphosphatase''' | '''Crystal strucure of fructose-1,6-bisphosphatase''' | ||
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[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: | [[Category: Alpha-beta-beta-alpha four layer sandwich]] | ||
[[Category: | [[Category: Fructose-1,6-bisphosphatase]] | ||
[[Category: | [[Category: Hyperthermophilic archaea]] | ||
[[Category: | [[Category: Magnesium ion]] | ||
[[Category: | [[Category: Octamer]] | ||
[[Category: | [[Category: Phosphatase]] | ||
[[Category: | [[Category: Three metal-assisted mechanism]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:40 2008'' | |||
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Revision as of 08:25, 3 May 2008
Crystal strucure of fructose-1,6-bisphosphatase
Overview
As the first structure of the novel class of fructose-1,6-bisphosphatase (FBPase) present in thermophilic archaea, we solved the crystal structure of the ST0318 gene product (St-Fbp) of Sulfolobus tokodaii strain 7. The St-Fbp structure comprises a homooctamer of the 422 point-group. The protein folds as a four-layer alpha-beta-beta-alpha sandwich with a novel topology, which is completely different from the sugar phosphatase fold. The structure contains an unhydrolyzed FBP molecule in the open-keto form, as well as four hexacoordinated magnesium ions around the 1-phosphoryl group of FBP. The arrangement of the catalytic side chains and metal ligands is consistent with the three-metal ion assisted catalysis proposed for conventional FBPases. The structure provides an insight into the structural basis of the strict substrate specificity of St-Fbp.
About this Structure
1UMG is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.
Reference
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:15274916 Page seeded by OCA on Sat May 3 11:25:40 2008
