1umi: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1umi.jpg|left|200px]] | [[Image:1umi.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1umi", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1umi| PDB=1umi | SCENE= }} | |||
}} | |||
'''Structural basis of sugar-recognizing ubiquitin ligase''' | '''Structural basis of sugar-recognizing ubiquitin ligase''' | ||
| Line 37: | Line 34: | ||
[[Category: Yamaguchi, Y.]] | [[Category: Yamaguchi, Y.]] | ||
[[Category: Yoshida, Y.]] | [[Category: Yoshida, Y.]] | ||
[[Category: | [[Category: Lectin]] | ||
[[Category: | [[Category: Riken structural genomics/proteomics initiative]] | ||
[[Category: | [[Category: Rsgi]] | ||
[[Category: | [[Category: Scf]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Ubiquitin]] | ||
[[Category: | [[Category: Ubiquitin ligase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:39 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 08:25, 3 May 2008
Structural basis of sugar-recognizing ubiquitin ligase
Overview
SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.
About this Structure
1UMI is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:14990996 Page seeded by OCA on Sat May 3 11:25:39 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Mus musculus
- Single protein
- Ubiquitin--protein ligase
- Chiba, T.
- Hirao, T.
- Iwai, K.
- Kato, K.
- Lee, S J.
- Mizushima, T.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Tanaka, K.
- Tsukihara, T.
- Yamaguchi, Y.
- Yoshida, Y.
- Lectin
- Riken structural genomics/proteomics initiative
- Rsgi
- Scf
- Structural genomic
- Ubiquitin
- Ubiquitin ligase
