9bi0: Difference between revisions
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==Streptomyces griseus Family 2B encapsulin shell with 2-methylisoborneol synthase cargo in 20 mM cAMP== | |||
<StructureSection load='9bi0' size='340' side='right'caption='[[9bi0]], [[Resolution|resolution]] 2.93Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9bi0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus_subsp._griseus Streptomyces griseus subsp. griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BI0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.93Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bi0 OCA], [https://pdbe.org/9bi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bi0 RCSB], [https://www.ebi.ac.uk/pdbsum/9bi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bi0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q54255_STRGR Q54255_STRGR] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Terpenoids are the largest class of natural products, found across all domains of life. One of the most abundant bacterial terpenoids is the volatile odorant 2-methylisoborneol (2-MIB), partially responsible for the earthy smell of soil and musty taste of contaminated water. Many bacterial 2-MIB biosynthetic gene clusters were thought to encode a conserved transcription factor, named EshA in the model soil bacterium Streptomyces griseus. Here, we revise the function of EshA, now referred to as Sg Enc, and show that it is a Family 2B encapsulin shell protein. Using cryo-electron microscopy, we find that Sg Enc forms an icosahedral protein shell and encapsulates 2-methylisoborneol synthase (2-MIBS) as a cargo protein. Sg Enc contains a cyclic adenosine monophosphate (cAMP) binding domain (CBD)-fold insertion and a unique metal-binding domain, both displayed on the shell exterior. We show that Sg Enc CBDs do not bind cAMP. We find that 2-MIBS cargo loading is mediated by an N-terminal disordered cargo-loading domain and that 2-MIBS activity and Sg Enc shell structure are not modulated by cAMP. Our work redefines the function of EshA and establishes Family 2B encapsulins as cargo-loaded protein nanocompartments involved in secondary metabolite biosynthesis. | |||
The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell.,Andreas MP, Giessen TW Nat Commun. 2024 Nov 9;15(1):9715. doi: 10.1038/s41467-024-54175-4. PMID:39521781<ref>PMID:39521781</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9bi0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces griseus subsp. griseus]] | |||
[[Category: Andreas MP]] | |||
[[Category: Giessen TW]] | |||
Latest revision as of 06:19, 4 December 2024
Streptomyces griseus Family 2B encapsulin shell with 2-methylisoborneol synthase cargo in 20 mM cAMP
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