9f09: Difference between revisions

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'''Unreleased structure'''


The entry 9f09 is ON HOLD
==Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Complex with 2-deoxyribose, 7-Bromo-1H-imidazo[4,5-b]pyridine and 2'-deoxycytidine==
<StructureSection load='9f09' size='340' side='right'caption='[[9f09]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9f09]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_leichmannii Lactobacillus leichmannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9F09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9F09 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DR:2-DEOXY-BETA-D-ERYTHRO-PENTOFURANOSE'>2DR</scene>, <scene name='pdbligand=A1H8P:7-bromanyl-3~{H}-imidazo[4,5-b]pyridine'>A1H8P</scene>, <scene name='pdbligand=DCZ:2-DEOXYCYTIDINE'>DCZ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9f09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9f09 OCA], [https://pdbe.org/9f09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9f09 RCSB], [https://www.ebi.ac.uk/pdbsum/9f09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9f09 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NTD_LACLE NTD_LACLE] Catalyzes the cleavage of the glycosidic bond of 2'-deoxyribonucleosides and the transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base.<ref>PMID:7797550</ref> <ref>PMID:8805514</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nucleosides are pervasive building blocks that are found throughout nature and used extensively in medicinal chemistry and biotechnology. However, the preparation of base-modified analogues using conventional synthetic methodology poses challenges in scale-up and purification. In this work, an integrated approach involving structural analysis, screening and reaction optimization, is established to prepare 2'-deoxyribonucleoside analogues catalysed by the type II nucleoside 2'-deoxyribosyltransferase from Lactobacillus leichmannii (LlNDT-2). Structural analysis in combination with substrate profiling, identified the constraints on pyrimidine and purine acceptor bases by LlNDT2. A solvent screen identifies pure water as a suitable solvent for the preparation of high value purine and pyrimidine 2'-deoxyribonucleoside analogues on a gram scale under optimized reaction conditions. This approach provides the basis to establish a convergent, step-efficient chemoenzymatic platform for the preparation of high value 2'-deoxyribonucleosides.


Authors: Ascham, A., Salihovic, A., Burley, G., Grogan, G.
Gram-scale enzymatic synthesis of 2'-deoxyribonucleoside analogues using nucleoside transglycosylase-2.,Salihovic A, Ascham A, Taladriz-Sender A, Bryson S, Withers JM, McKean IJW, Hoskisson PA, Grogan G, Burley GA Chem Sci. 2024 Aug 27;15(37):15399-407. doi: 10.1039/d4sc04938a. PMID:39234214<ref>PMID:39234214</ref>


Description: Nucleoside-2''-deoxyribosyltransferase from Lactobacillus leichmannii. Complex with 2-deoxyribose, 7-Bromo-1H-imidazo[4,5-b]pyridine and 2''-deoxycytidine
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Ascham, A]]
<div class="pdbe-citations 9f09" style="background-color:#fffaf0;"></div>
[[Category: Burley, G]]
== References ==
[[Category: Salihovic, A]]
<references/>
[[Category: Grogan, G]]
__TOC__
</StructureSection>
[[Category: Lactobacillus leichmannii]]
[[Category: Large Structures]]
[[Category: Ascham A]]
[[Category: Burley G]]
[[Category: Grogan G]]
[[Category: Salihovic A]]
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